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(19)F-heptuloses as tools for the non-invasive imaging of GLUT2-expressing cells.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2012 Jan 15; Vol. 517 (2), pp. 138-43. Date of Electronic Publication: 2011 Nov 22. - Publication Year :
- 2012
-
Abstract
- Suitable analogs of d-mannoheptulose are currently considered as possible tools for the non-invasive imaging of pancreatic islet insulin-producing cells. Here, we examined whether (19)F-heptuloses could be used for non-invasive imaging of GLUT2-expressing cells. After 20 min incubation, the uptake of (19)F-heptuloses (25 mM) by rat hepatocytes, as assessed by (19)F NMR spectroscopy, ranged from 0.50 (1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose) to 0.25 (1,3-dideoxy-1,3-difluoro-d-mannoheptulose) and 0.13 (1-deoxy-1-fluoro-d-glucoheptulose, 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose) μmol per 3×10(6)cells. (19)F MRI experiments also allowed the detection of 1-deoxy-1-fluoro-d-mannoheptulose in rat hepatocytes. All three (19)F-mannoheptuloses cited above, as well as 7-deoxy-7-fluoro-d-mannoheptulose and 1-deoxy-1-fluoro-d-glucoheptulose inhibited insulin release evoked in rat isolated pancreatic islets by 10mM d-glucose to the same extent as that observed with an equivalent concentration (10mM) of d-mannoheptulose, while 3-deoxy-3-fluoro-d-glucoheptulose and 1,3-dideoxy-1,3-difluoro-d-glucoheptulose (also 10mM) were less potent than d-mannoheptulose in inhibiting insulin release. The 1-deoxy-1-fluoro-d-mannoheptulose and 3-deoxy-3-fluoro-d-mannoheptulose only marginally affected INS-1 cell viability. These findings are compatible with the view that selected (19)F-heptuloses may represent suitable tools for the non-invasive imaging of hepatocytes and insulin-producing cells by (19)F MRI.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cell Survival
Female
Fluorine chemistry
In Vitro Techniques
Insulin biosynthesis
Islets of Langerhans metabolism
Magnetic Resonance Imaging
Magnetic Resonance Spectroscopy
Mannoheptulose chemistry
Mannoheptulose pharmacokinetics
Phantoms, Imaging
Rats
Rats, Wistar
Glucose Transporter Type 2 metabolism
Hepatocytes metabolism
Mannoheptulose analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0384
- Volume :
- 517
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 22138222
- Full Text :
- https://doi.org/10.1016/j.abb.2011.11.014