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Alternative complement pathway of channel catfish (Ictalurus punctatus): molecular characterization, mapping and expression analysis of factors Bf/C2 and Df.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2012 Jan; Vol. 32 (1), pp. 186-95. Date of Electronic Publication: 2011 Nov 23. - Publication Year :
- 2012
-
Abstract
- The complement system is important in both innate and adaptive host defense against microbial infection in vertebrates. It contains three pathways: the classical, alternative, and lectin pathways. Complement component factors B and D are two crucial proteases in the alternative pathway. In this study, the genes of complement factors Bf/C2 and Df from channel catfish, Ictalurus punctatus were identified and characterized. Two complement factor B-related genes, Bf/C2A and Bf/C2B, and factor D gene Df were identified. Phylogenetic analysis suggested that Bf/C2A and Bf/C2B is likely orthologous to factor B and factor C2, respectively. Southern blot results suggested that these three genes are all single-copy genes in the catfish genome. The catfish Bf/C2A, Bf/C2B and Df genes were genetically mapped on linkage group 3, 20 and 29, respectively. Bf/C2A and Bf/C2B are highly expressed in liver and kidney, while Df is highly expressed in gill and spleen. After infection with Edwardsiella ictaluri, the expression of Bf/C2A, Bf/C2B and Df genes were found to be remarkably induced in the gill, liver, spleen and kidney at some sampling times, indicating that these three complement factors play a pivotal role in immune responses after the bacterial infection in catfish.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Enterobacteriaceae Infections immunology
Enterobacteriaceae Infections veterinary
Fish Diseases immunology
Gene Dosage
Gene Expression Profiling
Gene Order
Genetic Linkage
Ictaluridae classification
Phylogeny
Sequence Alignment
Complement Factor B genetics
Complement Factor B immunology
Complement Factor D genetics
Complement Factor D immunology
Complement Pathway, Alternative genetics
Complement Pathway, Alternative immunology
Gene Expression Regulation
Ictaluridae genetics
Ictaluridae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 32
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 22138130
- Full Text :
- https://doi.org/10.1016/j.fsi.2011.11.012