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Oxidative stress is a consequence, not a cause, of aluminum toxicity in the forage legume Lotus corniculatus.
- Source :
-
The New phytologist [New Phytol] 2012 Feb; Vol. 193 (3), pp. 625-636. Date of Electronic Publication: 2011 Dec 02. - Publication Year :
- 2012
-
Abstract
- • Aluminum (Al) toxicity is a major limiting factor of crop production on acid soils, but the implication of oxidative stress in this process is controversial. A multidisciplinary approach was used here to address this question in the forage legume Lotus corniculatus. • Plants were treated with low Al concentrations in hydroponic culture, and physiological and biochemical parameters, together with semiquantitative metabolic and proteomic profiles, were determined. • The exposure of plants to 10 μM Al inhibited root and leaf growth, but had no effect on the production of reactive oxygen species or lipid peroxides. By contrast, exposure to 20 μM Al elicited the production of superoxide radicals, peroxide and malondialdehyde. In response to Al, there was a progressive replacement of the superoxide dismutase isoforms in the cytosol, a loss of ascorbate and consistent changes in amino acids, sugars and associated enzymes. • We conclude that oxidative stress is not a causative factor of Al toxicity. The increased contents in roots of two powerful Al chelators, malic and 2-isopropylmalic acids, together with the induction of an Al-activated malate transporter gene, strongly suggest that both organic acids are implicated in Al detoxification. The effects of Al on key proteins involved in cytoskeleton dynamics, protein turnover, transport, methylation reactions, redox control and stress responses underscore a metabolic dysfunction, which affects multiple cellular compartments, particularly in plants exposed to 20 μM Al.<br /> (© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.)
- Subjects :
- Antioxidants metabolism
Carboxylic Acids metabolism
Gene Expression Regulation, Plant drug effects
Genes, Plant genetics
Lotus genetics
Lotus growth & development
Metabolomics
Nutritional Physiological Phenomena drug effects
Plant Leaves drug effects
Plant Leaves genetics
Plant Leaves metabolism
Plant Proteins genetics
Plant Proteins metabolism
Plant Roots cytology
Plant Roots drug effects
Plant Roots genetics
Plant Roots metabolism
Protein Isoforms metabolism
Proteome metabolism
Proteomics
RNA, Messenger genetics
RNA, Messenger metabolism
Reactive Oxygen Species metabolism
Stress, Physiological drug effects
Stress, Physiological genetics
Aluminum toxicity
Lotus drug effects
Lotus metabolism
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 193
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 22136521
- Full Text :
- https://doi.org/10.1111/j.1469-8137.2011.03978.x