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Interleukin-27 induces interferon-inducible genes: analysis of gene expression profiles using Affymetrix microarray and DAVID.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 820, pp. 25-53. - Publication Year :
- 2012
-
Abstract
- We have previously demonstrated that IL-27 is a novel anti-HIV cytokine, which inhibits HIV replication in CD4 T cells and macrophages as interferon (IFN)-α does. To further understand the mechanism of the antiviral effect, we performed Affymetrix DNA microarray and gene functional annotation analysis using DAVID (the Database for Annotation, Visualization, and Integrated Discovery). DAVID is a web-based bioinformatics application that systematically identifies enriched biology associated with large gene list(s) derived from high-throughput genomic experiments, such as microarray. The enriched annotation terms identified by DAVID will give important insights into understanding the biological themes under study. Having used the DAVID bioinformatics tools, we have shown that IL-27 differentially regulates the gene expression between T cells and macrophages. IL-27 significantly induces IFN-inducible genes including antiviral genes in macrophages as does IFN-α, suggesting that IL-27 inhibits HIV replication in macrophages via a mechanism similar to that of IFN-α.
- Subjects :
- CD4 Lymphocyte Count
Cell Differentiation
Computational Biology methods
Databases, Genetic
Gene Expression drug effects
Gene Expression Profiling methods
HIV physiology
Humans
Internet
Macrophages metabolism
Pilot Projects
RNA isolation & purification
T-Lymphocytes metabolism
Virus Replication
Interferon Inducers metabolism
Interleukin-17 metabolism
Oligonucleotide Array Sequence Analysis methods
Software
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 820
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22131024
- Full Text :
- https://doi.org/10.1007/978-1-61779-439-1_3