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Collection and storage requirements for urinary kidney injury molecule-1 (KIM-1) measurements in humans.
- Source :
-
Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2011 Nov 23; Vol. 50 (3), pp. 539-43. Date of Electronic Publication: 2011 Nov 23. - Publication Year :
- 2011
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Abstract
- Background: Urinary kidney injury molecule-1 (KIM-1) is a recently discovered biomarker for early renal damage. However, little is known about the collection and storage requirements prior to its measurement in human urine.<br />Methods: Samples of healthy volunteers were collected and aliquoted. The effect of pre-freezing time, thawing, addition of protease inhibitors, centrifugation, storage time (up to 1.5 years) and temperature (4°C, -20°C and -80°C) was tested.<br />Results: Addition of protease inhibitors and centrifugation prior to freezing did not affect the KIM-1 measurements. When samples were kept at room temperature for longer than 3 h before freezing or defrosted more than 1 h before measurement, mean KIM-1 values differed significantly compared to aliquots with minimal pre-freezing and thawing time. Samples frozen at -80°C were stable for up to 1.5 years; however an increasing number of freeze-thaw cycles adversely affected KIM-1 measurements. When stored at 4°C and -20°C, samples were less stable compared to those stored at -80°C.<br />Conclusions: This study recommends that urine samples collected for KIM-1 measurements are frozen within 3 h after voiding and only be defrosted immediately prior to measurement. Addition of protease inhibitor and centrifugation prior to measurement is not necessary. Samples are preferably stored at -80°C and freeze-thaw cycles should be avoided.
Details
- Language :
- English
- ISSN :
- 1437-4331
- Volume :
- 50
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Clinical chemistry and laboratory medicine
- Publication Type :
- Academic Journal
- Accession number :
- 22107138
- Full Text :
- https://doi.org/10.1515/CCLM.2011.796