Back to Search
Start Over
Unsaturated fatty acids induce mesenchymal stem cells to increase secretion of angiogenic mediators.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2012 Sep; Vol. 227 (9), pp. 3225-33. - Publication Year :
- 2012
-
Abstract
- Mesenchymal stem cells (MSC) represent emerging cell-based therapies for diabetes and associated complications. Ongoing clinical trials are using exogenous MSC to treat type 1 and 2 diabetes, cardiovascular disease and non-healing wounds due to diabetes. The majority of these trials are aimed at exploiting the ability of these multipotent mesenchymal stromal cells to release soluble mediators that reduce inflammation and promote both angiogenesis and cell survival at sites of tissue damage. Growing evidence suggests that MSC secretion of soluble factors is dependent on tissue microenvironment. Despite the contribution of fatty acids to the metabolic environment of type 2 diabetes, almost nothing is known about their effects on MSC secretion of growth factors and cytokines. In this study, human bone marrow-derived MSC were exposed to linoleic acid, an omega-6 polyunsaturated fatty acid, or oleic acid, a monounsaturated fatty acid, for seven days in the presence of 5.38 mM glucose. Outcomes measured included MSC proliferation, gene expression, protein secretion and chemotaxis. Linoleic and oleic acids inhibited MSC proliferation and altered MSC expression and secretion of known mediators of angiogenesis. Both unsaturated fatty acids induced MSC to increase secretion of interleukin-6, VEGF and nitric oxide. In addition, linoleic acid but not oleic acid induced MSC to increase production of interleukin-8. Collectively these data suggest that exposure to fatty acids may have functional consequences for MSC therapy. Fatty acids may affect MSC engraftment to injured tissue and MSC secretion of cytokines and growth factors that regulate local cellular responses to injury.<br /> (Copyright © 2011 Wiley Periodicals, Inc.)
- Subjects :
- Angiogenic Proteins genetics
Bone Marrow drug effects
Cell Count
Cell Proliferation drug effects
Cell- and Tissue-Based Therapy methods
Chemotaxis drug effects
Gene Expression drug effects
Glucose pharmacology
Humans
Mesenchymal Stem Cells drug effects
Nitric Oxide metabolism
Angiogenic Proteins metabolism
Bone Marrow metabolism
Linoleic Acid pharmacology
Mesenchymal Stem Cells metabolism
Oleic Acid pharmacology
Wound Healing
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 227
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 22105830
- Full Text :
- https://doi.org/10.1002/jcp.24013