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MiR-17-92 cluster regulates cell proliferation and collagen synthesis by targeting TGFB pathway in mouse palatal mesenchymal cells.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2012 Apr; Vol. 113 (4), pp. 1235-44. - Publication Year :
- 2012
-
Abstract
- Elongation and elevation of palatal shelves, mainly caused by proliferation and extra-cellular matrix synthesis of palatal mesenchymal cells (PMCs), are essential for normal palatal development. Transforming growth factor beta (TGFB) pathway could induce proliferation inhibition and collagen synthesis in PMCs. Recent studies found that miRNA-17-92 (miR-17-92) cluster, including miR-17, miR-18a, miR-19a, miR-20a, miR-19b, and miR-92a, expressed in the 1st bronchial arch of mouse embryos during the period of palatal shelf elongation and elevation, and directly targeted TGFB pathway in cancer cell lines. Whether miR-17-92 cluster expresses and targets TGFB pathway in PMCs has not yet been studied. Using quantitative real-time RT-PCR, we found that miR-17-92 expressed in PMCs and decreased from embryonic day (E) 12 to E14 in palatal shelves. MTT assay and Western blot showed that miR-17-92 inhibited TGFB1 induced proliferation inhibition and collagen synthesis in PMCs by decreasing TGFBR2, SMAD2, and SMAD4 protein level. Further luciferase assay showed that miR-17 and miR-20a directly targeted 3′UTR of TGFBR2, and that miR-18a directly targeted 3′UTR of SMAD2 and SMAD4. We thus conclude that miR-17-92 cluster could inhibit TGFB pathway induced proliferation inhibition and collagen synthesis in PMCs by directly targeting TGFBR2, SMAD2, and SMAD4.<br /> (© 2011 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Base Sequence
Blotting, Western
DNA Primers
Mice
Palate cytology
Palate embryology
Protein Serine-Threonine Kinases antagonists & inhibitors
Protein Serine-Threonine Kinases metabolism
Real-Time Polymerase Chain Reaction
Receptor, Transforming Growth Factor-beta Type II
Receptors, Transforming Growth Factor beta antagonists & inhibitors
Receptors, Transforming Growth Factor beta metabolism
Reverse Transcriptase Polymerase Chain Reaction
Smad2 Protein antagonists & inhibitors
Smad2 Protein metabolism
Smad4 Protein antagonists & inhibitors
Smad4 Protein metabolism
Cell Proliferation
Collagen biosynthesis
Mesoderm cytology
MicroRNAs physiology
Palate metabolism
Transforming Growth Factor beta physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4644
- Volume :
- 113
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 22095742
- Full Text :
- https://doi.org/10.1002/jcb.23457