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[Regulating action of iron regulatory protein-2 in iron metabolism of lung cancer].
- Source :
-
Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2011 Jul 26; Vol. 91 (28), pp. 1992-5. - Publication Year :
- 2011
-
Abstract
- Objective: To discuss the regulating mechanism of iron regulatory protein-2 (IRP2) in the iron metabolism of lung cancer.<br />Methods: The cultured A549 cells were divided into 3 groups: liposome group (including liposomes 20 mg/L), random oligonucleotide group (SCODN group) and antisense oligonucleotide group (ASODN group). And the liposome-mediated transfection was employed with the liposome and SCODN groups as controls. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to examine the mRNA and protein expressions of iron metabolism-related transferring (Tf), transferrin receptor (TfR) and ferritin (Fn) genes, etc.<br />Results: After a 48-hour transfection, the mRNA expression of Tf had no statistically significant difference among three groups (F = 2.18, P = 0.078); the mRNA expression of TfR in the ASODN group was significantly lower than that in the liposome and SCODN groups (P < 0.05). The expression of Fn mRNA in the ASODN group (0.56 ± 0.06) was higher than that in the liposome (0.36 ± 0.05) and SCODN groups (0.39 ± 0.03) (P < 0.05). After a 48-hour transfection, the IRP2 protein expression of the ASODN group was significantly lower than those of the liposome and SCODN groups (P < 0.05). The Tf protein expression was not statistically different in three groups (F = 2.67, P = 0.088). The TfR protein expression of the ASODN group was lower than those of the liposome and SCODN groups (P < 0.05). And the Fn protein expression of the ASODN group was higher than those of the liposome and SCODN groups (P < 0.05).<br />Conclusion: IRP2 may affect the expressions of TfR and Fn in lung adenocarcinoma A549 cells by changing the amount of protein and regulating the iron metabolism.
- Subjects :
- Adenocarcinoma of Lung
Cell Line, Tumor
Ferritins metabolism
Humans
Iron Regulatory Protein 2 genetics
Liposomes administration & dosage
Oligodeoxyribonucleotides, Antisense genetics
Receptors, Transferrin metabolism
Adenocarcinoma metabolism
Iron metabolism
Iron Regulatory Protein 2 metabolism
Lung Neoplasms metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0376-2491
- Volume :
- 91
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- Zhonghua yi xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 22093897