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Characterization of supported lipid bilayer disruption by chrysophsin-3 using QCM-D.
- Source :
-
The journal of physical chemistry. B [J Phys Chem B] 2011 Dec 29; Vol. 115 (51), pp. 15228-35. Date of Electronic Publication: 2011 Dec 05. - Publication Year :
- 2011
-
Abstract
- Antimicrobial peptides (AMPs) are naturally occurring polymers that can kill bacteria by destabilizing their membranes. A quartz crystal microbalance with dissipation monitoring (QCM-D) was used to better understand the action of the AMP chrysophsin-3 on supported lipid bilayers (SLB) of phosphatidylcholine. Interaction of the SLB with chrysophsin-3 at 0.05 μM demonstrated changes in frequency (Δf) and energy dissipation (ΔD) that were near zero, indicating little change in the membrane. At higher concentrations of chyrsophsin-3 (0.25-4 μM), decreases in Δf of up to 7 Hz were measured. These negative frequency changes suggest that mass was being added to the SLB, possibly due to peptide insertion into the membrane. At a chrysophsin-3 concentration of 10 μM, there was a net mass loss, which was attributed to pore formation in the membrane. QCM-D can be used to describe a mechanistic relationship between AMP concentration and interaction with a model cell membrane.<br /> (© 2011 American Chemical Society)
Details
- Language :
- English
- ISSN :
- 1520-5207
- Volume :
- 115
- Issue :
- 51
- Database :
- MEDLINE
- Journal :
- The journal of physical chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 22085290
- Full Text :
- https://doi.org/10.1021/jp209658y