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Transfusion in the absence of inflammation induces antigen-specific tolerance to murine RBCs.
- Source :
-
Blood [Blood] 2012 Feb 09; Vol. 119 (6), pp. 1566-9. Date of Electronic Publication: 2011 Nov 10. - Publication Year :
- 2012
-
Abstract
- Most human transfusion recipients fail to make detectable alloantibodies to foreign RBC antigens ("nonresponders"). Herein, we use a murine model to test the hypothesis that nonresponders may be immunologically tolerant. FVB mice transfused with RBCs expressing transgenic human glycophorin A (hGPA) antigen in the absence of inflammation produced undetectable levels of anti-hGPA immunoglobulins, unlike those transfused in the presence of polyinosinic:polycytidylic acid-induced inflammation. Mice in the nonresponder group failed to produce anti-hGPA after subsequent transfusions in the presence of polyinosinic:polycytidylic acid, whereas anti-hGPA levels increased in the responder group. This tolerance was antigen specific, because nonresponders to hGPA produced alloantibodies to RBCs that expressed a different transgenic antigen. This tolerance was not an idiosyncrasy of the hGPA antigen nor of the recipient strain, because B10.BR mice transfused with membrane-bound hen egg lysozyme antigen-transgenic RBCs also demonstrated induced nonresponsiveness. These data demonstrate that RBCs transfused in the absence of inflammation can induce tolerance.
- Subjects :
- Analysis of Variance
Animals
Antibodies blood
Antibodies immunology
Antigens immunology
Enzyme-Linked Immunosorbent Assay
Erythrocytes metabolism
Female
Glycophorins genetics
Glycophorins immunology
Humans
Inflammation blood
Isoantibodies blood
Isoantibodies immunology
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Muramidase genetics
Muramidase immunology
Erythrocyte Transfusion methods
Erythrocytes immunology
Immune Tolerance immunology
Inflammation immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1528-0020
- Volume :
- 119
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Blood
- Publication Type :
- Academic Journal
- Accession number :
- 22077064
- Full Text :
- https://doi.org/10.1182/blood-2011-09-382655