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Ripe fruit of Rubus coreanus inhibits mast cell-mediated allergic inflammation.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2012 Feb; Vol. 29 (2), pp. 303-10. Date of Electronic Publication: 2011 Nov 07. - Publication Year :
- 2012
-
Abstract
- In this study, we investigated the effect of a water extract of the ripe fruits of Rubus coreanus Miq. (Rosaceae) (RFRC) on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, rhinitis, asthma and atopic dermatitis. RFRC dose-dependently inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. RFRC reduced the immunoglobulin E (IgE)-mediated local allergic reaction, passive cutaneous anaphylaxis. RFRC attenuated histamine release from rat peritoneal mast cells and human mast cells by the reduction of intracellular calcium. RFRC decreased the phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187 (PMACI)-stimulated expression and secretion of pro-inflammatory cytokines in human mast cells. The inhibitory effect of RFRC on cytokine production was nuclear factor (NF)-κB- and mitogen-activated protein kinase (MAPK)-dependent. In addition, RFRC suppressed the activation of caspase-1. Our findings provide evidence that RFRC inhibits mast cell-derived allergic inflammatory reactions, and for the involvement of calcium, NF-κB, MAPKs and caspase-1 in these effects. Furthermore, in vivo and in vitro anti-allergic inflammatory effects of RFRC provide affirmative proof of a possible therapeutic application of this agent in allergic inflammatory diseases.
- Subjects :
- Anaphylaxis chemically induced
Animals
Calcium metabolism
Caspase 1 metabolism
Cell Line
Disease Models, Animal
Enzyme Activation drug effects
Gene Expression drug effects
Histamine Release drug effects
Humans
Inflammation immunology
Inflammation Mediators metabolism
Male
Mice
Mitogen-Activated Protein Kinases metabolism
NF-kappa B metabolism
Rats
Rats, Sprague-Dawley
p-Methoxy-N-methylphenethylamine adverse effects
Anaphylaxis immunology
Fruit chemistry
Mast Cells drug effects
Mast Cells immunology
Plant Extracts pharmacology
Rosaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 22075758
- Full Text :
- https://doi.org/10.3892/ijmm.2011.829