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Enhanced gastric ghrelin production and secretion in rats with gastric outlet obstruction.
- Source :
-
Digestive diseases and sciences [Dig Dis Sci] 2012 Apr; Vol. 57 (4), pp. 858-64. Date of Electronic Publication: 2011 Nov 04. - Publication Year :
- 2012
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Abstract
- Background and Aim: Ghrelin has distinct effects on gastrointestinal motility through the vagus nerve and gastric excitatory neural plexus. The objectives of this study were to investigate the dynamics of ghrelin and expression of neuromuscular markers in a newly established surgically manipulated rat model of gastric outlet obstruction (GOO), akin to the pyloric stricture associated with duodenal ulcer, advanced gastric cancer, and other conditions, in the clinical setting.<br />Material and Methods: The rats were divided into two groups, a control group (sham operation) and the GOO group (proximal duodenal stricture). The animals were sacrificed 2 weeks after the operation. Plasma and gastric ghrelin were measured by radioimmunoassay. mRNA expression in the stomach of neural choline acetyltransferase (ChAT), c-kit, and membrane-bound stem cell factor (SCF) were analyzed by quantitative RT-PCR. In addition, gastric mRNA expression of the aforementioned were also evaluated 60 min after intraperitoneal administration of a synthetic GHS-R1a antagonist ([D: -Lys3] GHRP-6 6.0 mg/kg).<br />Results: Mechanical GOO induced increases of fasting plasma ghrelin levels and hyperplasia of the gastric muscle layers, with enhanced expression of the gastric neuromuscular markers. Administration of [D: -Lys3] GHRP-6 normalized the enhanced expression of c-kit and SCF.<br />Conclusion: GOO stimulates ghrelin dynamics and then enhances the mechanistic expression of gastric cellular communication network molecules between nerves and smooth muscle cells.
- Subjects :
- Animals
Choline O-Acetyltransferase metabolism
Food Deprivation physiology
Gastric Emptying
Gastric Outlet Obstruction pathology
Gastric Outlet Obstruction physiopathology
Ghrelin blood
Immunohistochemistry
Male
Oligopeptides metabolism
Oligopeptides pharmacology
Organ Size
Proto-Oncogene Proteins c-kit metabolism
Pyloric Stenosis metabolism
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Stem Cell Factor metabolism
Stomach innervation
Stomach pathology
Gastric Mucosa metabolism
Gastric Outlet Obstruction metabolism
Ghrelin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-2568
- Volume :
- 57
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Digestive diseases and sciences
- Publication Type :
- Academic Journal
- Accession number :
- 22052447
- Full Text :
- https://doi.org/10.1007/s10620-011-1953-y