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The hepatoprotective effects of adenine nucleotide translocator-2 against aging and oxidative stress.
- Source :
-
Free radical research [Free Radic Res] 2012 Jan; Vol. 46 (1), pp. 21-9. Date of Electronic Publication: 2011 Nov 22. - Publication Year :
- 2012
-
Abstract
- Mitochondrial adenine nucleotide translocator (ANT) plays important roles in the regulation of mitochondrial permeability transition and cell bioenergetics. The mouse has three ANT isoforms (1, 2 and 4) showing tissue-specific expression patterns. Although ANT1 is known to have a pro-apoptotic property, the specific functions of ANT2 have not been well determined. In the present study, ANT2 expression was significantly lower in the aged rat liver and in a liver fibrosis model. To explore the protective role of ANT2 in the liver, we established a hepa1c1c7 cell line overexpressing ANT2. Overexpression of ANT2 caused hepa1c1c7 cells to be more resistant to oxidative stress, and mitochondrial membrane potential (MMP, ∆Ψm) was relatively intact in ANT2-overexpressing cells under oxidative stress. In addition, ANT2 was found to increase ATP production by influencing mitochondrial bioenergetics. These results imply that the hepatoprotective effect of ANT2 is due to the stabilization of MMP and enhanced ATP production, and thus, maintaining ANT2 levels in the liver might be important to enhance resistance to aging and oxidative stress.<br /> (© 2012 Informa UK, Ltd.)
- Subjects :
- Adenine Nucleotide Translocator 2 biosynthesis
Adenine Nucleotide Translocator 2 genetics
Animals
Disease Models, Animal
Hepatocytes metabolism
Hepatocytes pathology
Humans
Liver Cirrhosis metabolism
Male
Mice
Mice, Inbred ICR
Mitochondria metabolism
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species metabolism
Specific Pathogen-Free Organisms
Transfection
Adenine Nucleotide Translocator 2 metabolism
Aging metabolism
Liver metabolism
Oxidative Stress physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2470
- Volume :
- 46
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 22034909
- Full Text :
- https://doi.org/10.3109/10715762.2011.636042