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Characterization, mapping, and distribution of the two XMRV parental proviruses.
- Source :
-
Journal of virology [J Virol] 2012 Jan; Vol. 86 (1), pp. 328-38. Date of Electronic Publication: 2011 Oct 26. - Publication Year :
- 2012
-
Abstract
- Xenotropic murine leukemia virus-related virus (XMRV) was previously reported to be associated with human prostate cancer and chronic fatigue syndrome. Our groups recently showed that XMRV was created through recombination between two endogenous murine retroviruses, PreXMRV-1 and PreXMRV-2, during the passaging of a prostate tumor xenograft in nude mice. Here, multiple approaches that led to the identification of PreXMRV-2, as well as the distribution of both parental proviruses among different mouse species, are described. The chromosomal loci of both proviruses were determined in the mouse genome, and integration site information was used to analyze the distribution of both proviruses in 48 laboratory mouse strains and 46 wild-derived strains. The strain distributions of PreXMRV-1 and PreXMRV-2 are quite different, the former being found predominantly in Asian mice and the latter in European mice, making it unlikely that the two XMRV ancestors could have recombined independently in the wild to generate an infectious virus. XMRV was not present in any of the mouse strains tested, and among the wild-derived mouse strains analyzed, not a single mouse carried both parental proviruses. Interestingly, PreXMRV-1 and PreXMRV-2 were found together in three laboratory strains, Hsd nude, NU/NU, and C57BR/cd, consistent with previous data that the recombination event that led to the generation of XMRV could have occurred only in the laboratory. The three laboratory strains carried the Xpr1(n) receptor variant nonpermissive to XMRV and xenotropic murine leukemia virus (X-MLV) infection, suggesting that the xenografted human tumor cells were required for the resulting XMRV recombinant to infect and propagate.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Chromosome Mapping
Female
Humans
Male
Mice genetics
Mice, Inbred Strains
Molecular Sequence Data
Proviruses isolation & purification
Proviruses physiology
Rats
Rats, Sprague-Dawley
Receptors, Virus chemistry
Receptors, Virus genetics
Recombination, Genetic
Retroviridae Infections virology
Sequence Alignment
Virus Integration
Xenotropic and Polytropic Retrovirus Receptor
Xenotropic murine leukemia virus-related virus isolation & purification
Xenotropic murine leukemia virus-related virus physiology
Mice virology
Proviruses genetics
Retroviridae Infections veterinary
Xenotropic murine leukemia virus-related virus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 86
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 22031947
- Full Text :
- https://doi.org/10.1128/JVI.06022-11