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Uropathogenic E. coli promote a paracellular urothelial barrier defect characterized by altered tight junction integrity, epithelial cell sloughing and cytokine release.
- Source :
-
Journal of comparative pathology [J Comp Pathol] 2012 Jul; Vol. 147 (1), pp. 11-9. Date of Electronic Publication: 2011 Oct 19. - Publication Year :
- 2012
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Abstract
- The urinary bladder is a common site of bacterial infection with a majority of cases attributed to uropathogenic Escherichia coli. Sequelae of urinary tract infections (UTIs) include the loss of urothelial barrier function and subsequent clinical morbidity secondary to the permeation of urine potassium, urea and ammonia into the subepithelium. To date there has been limited research describing the mechanism by which this urothelial permeability defect develops. The present study models acute uropathogenic E. coli infection in vitro using intact canine bladder mucosa mounted in Ussing chambers to determine whether infection induces primarily a transcellular or paracellular permeability defect. The Ussing chamber sustains tissue viability while physically separating submucosal and lumen influences, so this model is ideal for quantitative measurement of transepithelial electrical resistance (TER) to assess alterations of urothelial barrier function. Using this model, changes in both tissue ultrastructure and TER indicated that uropathogenic E. coli infection promotes a paracellular permeability defect associated with the failure of umbrella cell tight junction formation and umbrella cell sloughing. In addition, bacterial interaction with the urothelium promoted secretion of cytokines from the urinary bladder with bioactivity capable of modulating epithelial barrier function including tumour necrosis factor-α, interleukin (IL)-6 and IL-15. IL-15 secretion by the infected bladder mucosa is a novel finding and, because IL-15 plays key roles in reconstitution of tight junction function in damaged intestine, this study points to a potential role for IL-15 in UTI-induced urothelial injury.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Membrane Permeability physiology
Diffusion Chambers, Culture
Dogs
Electric Conductivity
Epithelial Cells metabolism
Epithelial Cells microbiology
Tight Junctions ultrastructure
Tissue Culture Techniques
Urinary Bladder metabolism
Urinary Bladder microbiology
Urinary Bladder pathology
Urothelium metabolism
Urothelium microbiology
Cytokines metabolism
Epithelial Cells pathology
Tight Junctions pathology
Uropathogenic Escherichia coli physiology
Urothelium pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-3129
- Volume :
- 147
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of comparative pathology
- Publication Type :
- Academic Journal
- Accession number :
- 22014415
- Full Text :
- https://doi.org/10.1016/j.jcpa.2011.09.005