Kinetic capillary electrophoresis with mass-spectrometry detection (KCE-MS) facilitates label-free solution-based kinetic analysis of protein-small molecule binding.

Authors :: Bao J
Krylova SM
Wilson DJ
Reinstein O
Johnson PE
Krylov SN
Source :: Chembiochem : a European journal of chemical biology [Chembiochem] 2011 Nov 25; Vol. 12 (17), pp. 2551-4. Date of Electronic Publication: 2011 Oct 19.
Publication Year :: 2011
Abstract: Tandem tracker: Here we introduce a method for studying the kinetics of protein-small-molecule interactions based on kinetic capillary electrophoresis (KCE) separation and MS detection. Due to the variety of KCE methods and MS modes available, the KCE-MS tandem is a highly versatile platform for label-free, solution-based kinetic studies of affinity interactions.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Alprenolol chemistry
Alprenolol metabolism
Kinetics
Labetalol chemistry
Labetalol metabolism
Orosomucoid chemistry
Orosomucoid metabolism
Pindolol chemistry
Pindolol metabolism
Propranolol chemistry
Propranolol metabolism
Protein Binding
Proteins chemistry
Small Molecule Libraries chemistry
Electrophoresis, Capillary
Mass Spectrometry
Proteins metabolism
Small Molecule Libraries metabolism

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