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Ectopic osteogenesis of mouse bone marrow stromal cells transfected with BMP 2/VEGF(165) genes in vivo.
- Source :
-
Orthopaedic surgery [Orthop Surg] 2009 Nov; Vol. 1 (4), pp. 322-5. - Publication Year :
- 2009
-
Abstract
- Objective: To evaluate the osteogenic efficacy of mouse bone marrow stromal cells (mBMSC) transfected with bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor 165 (VEGF(165)) genes.<br />Methods: pIRES-BMP2-VEGF(165) plasmid DNA was introduced into the mBMSC using a liposome-mediated method. The expression of BMP2 and VEGF(165) genes was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis. Transfected cells were injected into the thigh muscle pouches of four nude mice. The osteoinductivity activity of the transfected cells was evaluated by radiographic and histological analysis at 4 weeks after injection.<br />Results: The mRNA and proteins of both BMP2 and VEGF(165) were successfully expressed in mBMSC as confirmed by RT-PCR and immunohistochemical analysis. Ectopically formed bone tissue was clearly observed at 4 weeks after cell injection in the thigh muscle pouches of the nude mice.<br />Conclusion: mBMSC transfected with BMP2 and VEGF(165) genes can induce ectopic osteogenesis.<br /> (© 2009 Tianjin Hospital and Blackwell Publishing Asia Pty Ltd.)
- Subjects :
- Animals
Bone Marrow Cells metabolism
Cells, Cultured
Disease Models, Animal
Mice
Mice, Nude
Ossification, Heterotopic genetics
Ossification, Heterotopic metabolism
Ossification, Heterotopic pathology
Reverse Transcriptase Polymerase Chain Reaction
Stromal Cells metabolism
Transfection methods
Bone Marrow Cells cytology
Bone Morphogenetic Protein 2 genetics
Gene Expression Regulation
Osteogenesis genetics
RNA, Messenger genetics
Stromal Cells cytology
Vascular Endothelial Growth Factor A genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1757-7861
- Volume :
- 1
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Orthopaedic surgery
- Publication Type :
- Academic Journal
- Accession number :
- 22009883
- Full Text :
- https://doi.org/10.1111/j.1757-7861.2009.00045.x