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Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion.

Authors :
de Oliveira Silva F
das Neves Santos P
de Melo CM
Teixeira EH
de Sousa Cavada B
Arruda FV
Cajazeiras JB
Almeida AC
Pereira VA
Porto AL
Source :
Cell and tissue research [Cell Tissue Res] 2011 Nov; Vol. 346 (2), pp. 237-44. Date of Electronic Publication: 2011 Oct 19.
Publication Year :
2011

Abstract

Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.

Details

Language :
English
ISSN :
1432-0878
Volume :
346
Issue :
2
Database :
MEDLINE
Journal :
Cell and tissue research
Publication Type :
Academic Journal
Accession number :
22009293
Full Text :
https://doi.org/10.1007/s00441-011-1239-x