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Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion.
- Source :
-
Cell and tissue research [Cell Tissue Res] 2011 Nov; Vol. 346 (2), pp. 237-44. Date of Electronic Publication: 2011 Oct 19. - Publication Year :
- 2011
-
Abstract
- Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.
- Subjects :
- Animals
Cell Proliferation drug effects
Cell Survival drug effects
Cells, Cultured
Concanavalin A pharmacology
Interferon-gamma biosynthesis
Interleukin-10 biosynthesis
Interleukin-2 biosynthesis
Interleukin-6 biosynthesis
Male
Mice
Mice, Inbred BALB C
Nitric Oxide metabolism
Spleen drug effects
Adjuvants, Immunologic pharmacology
Cytokines metabolism
Immunization
Plant Lectins pharmacology
Spleen cytology
Spleen metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0878
- Volume :
- 346
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cell and tissue research
- Publication Type :
- Academic Journal
- Accession number :
- 22009293
- Full Text :
- https://doi.org/10.1007/s00441-011-1239-x