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Molecular identification tools for sibling species of Scedosporium and Pseudallescheria.
- Source :
-
Medical mycology [Med Mycol] 2012 Jul; Vol. 50 (5), pp. 497-508. Date of Electronic Publication: 2011 Oct 17. - Publication Year :
- 2012
-
Abstract
- The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 ß-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was less detailed. ITS was sufficient for the differentiation of most haplotypes of clinically relevant species (P. apiosperma, P. boydii, S. aurantiacum, S. dehoogii, and S. prolificans) and of environmental species (P. minutispora and Lophotrichus fimeti) when Restriction Fragment Length Polymorphism (RFLP) were applied. For the identification of P. apiosperma and P. boydii species-specific BT2 primers were needed. Pseudallescheria fusoidea, P. ellipsoidea and P. angusta remained difficult to distinguish from P. boydii.
- Subjects :
- DNA Primers
DNA, Fungal analysis
DNA, Fungal genetics
DNA, Ribosomal Spacer genetics
Humans
Mycoses diagnosis
Phylogeny
Polymorphism, Restriction Fragment Length
Pseudallescheria classification
Scedosporium classification
Sequence Analysis, DNA
Species Specificity
Tubulin genetics
DNA, Ribosomal Spacer analysis
Mycological Typing Techniques methods
Mycoses microbiology
Pseudallescheria genetics
Scedosporium genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2709
- Volume :
- 50
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Medical mycology
- Publication Type :
- Academic Journal
- Accession number :
- 22004363
- Full Text :
- https://doi.org/10.3109/13693786.2011.618939