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Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Oct 28; Vol. 414 (3), pp. 625-30. Date of Electronic Publication: 2011 Oct 06. - Publication Year :
- 2011
-
Abstract
- Arginine analogs were incorporated site-specifically into proteins using an in vitro translation system. In this system, mRNAs containing a CGGG codon were translated by an aminoacyl-tRNA(CCCG), which was charged with arginine analogs using yeast arginyl-tRNA synthetase. N(G)-monomethyl-L-arginine, L-citrulline and L-homoarginine were incorporated successfully into proteins using this method. The influence of arginine monomethylation in histone H3 on the acetylation of lysine residues by histone acetyltransferase hGCN5 was investigated, and the results demonstrated that K9 acetylation was suppressed by the methylation of R8 and R17 but not by R26 methylation. K18 acetylation was not affected by the methylation of R8, R17 and R26. This site-specific modification strategy provides a way to explore the roles of post-translational modifications in the absence of heterogeneity due to other modifications.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Aminoacylation
Arginine genetics
Catalysis
Citrulline chemistry
Citrulline genetics
Homoarginine chemistry
Homoarginine genetics
Methylation
Molecular Sequence Data
omega-N-Methylarginine chemistry
omega-N-Methylarginine genetics
Arginine analogs & derivatives
Arginine-tRNA Ligase chemistry
Protein Biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 414
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 22001918
- Full Text :
- https://doi.org/10.1016/j.bbrc.2011.09.137