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Interleukin 1 beta increases the cytosolic free sodium concentration in isolated rat islets of Langerhans.
- Source :
-
Scandinavian journal of immunology [Scand J Immunol] 1990 Jul; Vol. 32 (1), pp. 53-8. - Publication Year :
- 1990
-
Abstract
- Interleukin 1 (IL-1) exerts both stimulatory and inhibitory (cytotoxic) effects on insulin-producing beta cells in isolated pancreatic islets. Since alteration in ion fluxes is crucial for endocrine cell activation and is a denominator of cell death, and since IL-1 was recently shown to increase the total sodium content in a murine pre-B-lymphocyte cell line, we investigated the effect of recombinant human IL-1 beta (rhIL-1 beta) on the cytosolic free sodium concentration (fNa+i) in rat islets. Furthermore, long-term rhIL-1 beta effects on islet cell function were studied during exposure of islets to amiloride, a blocker of the plasma membrane Na+/H+ exchange. One hour of islet exposure to 60 U/ml of rhIL-1 beta caused a threefold increase in fNa+i in islet cells, and this effect was abolished by depletion of extracellular sodium. Blockade of Na+/H+ exchange with amiloride abolished the inhibitory effect of rhIL-1 beta on insulin release. In conclusion, rhIL-1 beta was found to increase sodium influx in pancreatic islet cells. This might underlie the widespread effects of rhIL-1 beta on beta-cell function and morphology, possibly related to IL-1-mediated toxic free radical formation.
- Subjects :
- Amiloride pharmacology
Animals
DNA analysis
Dose-Response Relationship, Drug
Free Radicals
In Vitro Techniques
Insulin metabolism
Insulin Secretion
Islets of Langerhans analysis
Oxygen metabolism
Rats
Recombinant Proteins pharmacology
Sodium metabolism
Interleukin-1 pharmacology
Islets of Langerhans drug effects
Sodium analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0300-9475
- Volume :
- 32
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scandinavian journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2200107
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1990.tb02891.x