Back to Search
Start Over
Surface-modified ZnSe waveguides for label-free infrared attenuated total reflection detection of DNA hybridization.
- Source :
-
The Analyst [Analyst] 2011 Dec 07; Vol. 136 (23), pp. 4906-11. Date of Electronic Publication: 2011 Oct 13. - Publication Year :
- 2011
-
Abstract
- This communication presents a novel label-free biosensing method to monitor DNA hybridization via infrared attenuated total reflection (IR-ATR) spectroscopy using surface-modified ZnSe waveguides. Well-defined carboxyl-terminated monolayers were formed at H-terminated ZnSe by direct photochemical activation. Chemical activation of the acidic function was obtained by using succinimide/carbodiimide linkers. The sequential surface modification reactions were characterized by XPS and IR-ATR spectroscopy. Finally, a single stranded DNA probe with a C6-NH(2) 5' modifier was coupled to the ester-terminated surface via peptide bonding, and the hybridization of the immobilized DNA sequence with its complementary strand was directly evaluated by IR-ATR spectroscopy in the mid-infrared (MIR) spectral regime (3-20 μm) without requiring an additional label. A shift of the vibrational modes corresponding to the phosphodiester and deoxyribose structures of the DNA backbone was observed. Hence, this approach substantiates a novel strategy for label-free DNA detection utilizing mid-infrared spectroscopy as the optical sensing platform.
Details
- Language :
- English
- ISSN :
- 1364-5528
- Volume :
- 136
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- The Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 21994915
- Full Text :
- https://doi.org/10.1039/c0an00504e