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Immune and inflammatory gene signature in rat cerebrum in subarachnoid hemorrhage with microarray analysis.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2012 Jan; Vol. 5 (1), pp. 118-25. Date of Electronic Publication: 2011 Oct 03. - Publication Year :
- 2012
-
Abstract
- Cerebral vasospasm following subarachnoid hemorrhage (SAH) has been studied in terms of a contraction of the major cerebral arteries, but the effect of cerebrum tissue in SAH is not yet well understood. To gain insight into the biology of SAH-expressing cerebrum, we employed oligonucleotide microarrays to characterize the gene expression profiles of cerebrum tissue at the early stage of SAH. Functional gene expression in the cerebrum was analyzed 2 h following stage 1-hemorrhage in Sprague-Dawley rats. mRNA was investigated by performing microarray and quantitative real-time PCR analyses, and protein expression was determined by Western blot analysis. In this study, 18 upregulated and 18 downregulated genes displayed at least a 1.5-fold change. Five genes were verified by real-time PCR, including three upregulated genes [prostaglandin E synthase (PGES), CD14 antigen, and tissue inhibitor of metalloproteinase 1 (TIMP1)] as well as two downregulated genes [KRAB-zinc finger protein-2 (KZF-2) and γ-aminobutyric acid B receptor 1 (GABA B receptor)]. Notably, there were functional implications for the three upregulated genes involved in the inflammatory SAH process. However, the mechanisms leading to decreased KZF-2 and GABA B receptor expression in SAH have never been characterized. We conclude that oligonucleotide microarrays have the potential for use as a method to identify candidate genes associated with SAH and to provide novel investigational targets, including genes involved in the immune and inflammatory response. Furthermore, understanding the regulation of MMP9/TIMP1 during the early stages of SAH may elucidate the pathophysiological mechanisms in SAH rats.
- Subjects :
- Animals
Cerebrum metabolism
Gene Expression Profiling
Inflammation immunology
Intramolecular Oxidoreductases genetics
Intramolecular Oxidoreductases metabolism
Lipopolysaccharide Receptors genetics
Lipopolysaccharide Receptors metabolism
Male
Matrix Metalloproteinase 9 metabolism
Prostaglandin-E Synthases
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Receptors, GABA-B genetics
Receptors, GABA-B metabolism
Repressor Proteins genetics
Repressor Proteins metabolism
Subarachnoid Hemorrhage immunology
Tissue Inhibitor of Metalloproteinase-1 genetics
Tissue Inhibitor of Metalloproteinase-1 metabolism
Cerebrum immunology
Inflammation genetics
Oligonucleotide Array Sequence Analysis
Subarachnoid Hemorrhage genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 5
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 21971956
- Full Text :
- https://doi.org/10.3892/mmr.2011.614