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Development of an animal model for radiofrequency ablation of primary, virally induced hepatocellular carcinoma in the woodchuck.
- Source :
-
Journal of vascular and interventional radiology : JVIR [J Vasc Interv Radiol] 2011 Nov; Vol. 22 (11), pp. 1613-1618.e1. Date of Electronic Publication: 2011 Sep 29. - Publication Year :
- 2011
-
Abstract
- Purpose: To develop a consistent and reproducible method in an animal model for studies of radiofrequency (RF) ablation of primary hepatocellular carcinoma (HCC).<br />Materials and Methods: Fifteen woodchucks were inoculated with woodchuck hepatitis virus (WHV) to establish chronic infections. When serum γ-glutamyl transpeptidase levels became elevated, the animals were evaluated with ultrasound, and, in most cases, preoperative magnetic resonance (MR) imaging to confirm tumor development. Ultimately, RF ablation of tumors was performed by using a 1-cm probe with the animal submerged in a water bath for grounding. Ablation effectiveness was evaluated with contrast-enhanced MR imaging and gross and histopathologic analysis.<br />Results: RF ablation was performed in 15 woodchucks. Modifications were made to the initial study design to adapt methodology for the woodchuck. The last 10 of these animals were treated with a standardized protocol using a 1-cm probe that produced a consistent area of tumor necrosis (mean size of ablation, 10.2 mm × 13.1 mm) and led to no complications.<br />Conclusions: A safe, reliable and consistent method was developed to study RF ablation of spontaneous primary HCC using chronically WHV-infected woodchucks, an animal model of hepatitis B virus-induced HCC.<br /> (Copyright © 2011 SIR. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Biopsy
Carcinoma, Hepatocellular pathology
Carcinoma, Hepatocellular virology
Contrast Media
Equipment Design
Hepatitis B complications
Liver Neoplasms, Experimental pathology
Liver Neoplasms, Experimental virology
Magnetic Resonance Imaging
Marmota
Necrosis
Reproducibility of Results
Time Factors
Carcinoma, Hepatocellular surgery
Catheter Ablation instrumentation
Hepatitis B virology
Hepatitis B Virus, Woodchuck pathogenicity
Liver Neoplasms, Experimental surgery
Subjects
Details
- Language :
- English
- ISSN :
- 1535-7732
- Volume :
- 22
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of vascular and interventional radiology : JVIR
- Publication Type :
- Academic Journal
- Accession number :
- 21959057
- Full Text :
- https://doi.org/10.1016/j.jvir.2011.08.020