Back to Search
Start Over
Two-photon fluorescence vascular bioimaging with new bioconjugate probes selective toward the vascular endothelial growth factor receptor 2.
- Source :
-
Bioconjugate chemistry [Bioconjug Chem] 2011 Oct 19; Vol. 22 (10), pp. 2060-71. Date of Electronic Publication: 2011 Oct 04. - Publication Year :
- 2011
-
Abstract
- We report the synthesis and characterization of two amine reactive fluorescent dyes with efficient two-photon absorption (2PA) properties and high fluorescence quantum yields. Bioconjugation of these dyes with the DC-101 antibody proved to be useful for selectively imaging the vascular endothelial growth factor receptor 2 (VEGFR-2) in cells expressing this receptor in vitro and in "whole" mounted excised tumors (ex vivo) by two-photon fluorescence microscopy (2PFM). The penetration depths reached within the tumors by 2PFM was over 800 μm. In addition, the concentration of dye required for incubation of these bioconjugates was in the picomolar domain, the probes possessed very good photostability, and the 2PFM setup did not require any additional means of increasing the collection efficiencies of fluorescent photons to achieve the relatively deep tissue imaging that was realized, due, in large part, to the favorable photophysical properties of the new probes.
- Subjects :
- Animals
Antibodies, Monoclonal immunology
Cell Line
Humans
Immunoconjugates immunology
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Models, Molecular
Molecular Imaging
Swine
Vascular Endothelial Growth Factor Receptor-2 immunology
Antibodies, Monoclonal chemistry
Carcinoma, Lewis Lung diagnosis
Fluorescent Dyes chemistry
Immunoconjugates chemistry
Vascular Endothelial Growth Factor Receptor-2 analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1520-4812
- Volume :
- 22
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Bioconjugate chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21950485
- Full Text :
- https://doi.org/10.1021/bc200299z