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Desmosome dynamics in migrating epithelial cells requires the actin cytoskeleton.
- Source :
-
Experimental cell research [Exp Cell Res] 2011 Dec 10; Vol. 317 (20), pp. 2814-22. Date of Electronic Publication: 2011 Sep 16. - Publication Year :
- 2011
-
Abstract
- Re-modeling of epithelial tissues requires that the cells in the tissue rearrange their adhesive contacts in order to allow cells to migrate relative to neighboring cells. Desmosomes are prominent adhesive structures found in a variety of epithelial tissues that are believed to inhibit cell migration and invasion. Mechanisms regulating desmosome assembly and stability in migrating cells are largely unknown. In this study we established a cell culture model to examine the fate of desmosomal components during scratch wound migration. Desmosomes are rapidly assembled between epithelial cells at the lateral edges of migrating cells and structures are transported in a retrograde fashion while the structures become larger and mature. Desmosome assembly and dynamics in this system are dependent on the actin cytoskeleton prior to being associated with the keratin intermediate filament cytoskeleton. These studies extend our understanding of desmosome assembly and provide a system to examine desmosome assembly and dynamics during epithelial cell migration.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Actin Cytoskeleton metabolism
Cell Adhesion physiology
Cell Line, Tumor
Cytoskeletal Proteins metabolism
Desmocollins metabolism
Desmosomes metabolism
Epithelial Cells cytology
Epithelial Cells metabolism
Humans
Intermediate Filaments metabolism
Keratins metabolism
Actin Cytoskeleton physiology
Cell Movement physiology
Desmosomes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2422
- Volume :
- 317
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 21945137
- Full Text :
- https://doi.org/10.1016/j.yexcr.2011.09.003