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Adaptation of the glycerol-3-phosphate dehydrogenase Gpd1 to high salinities in the extremely halotolerant Hortaea werneckii and halophilic Wallemia ichthyophaga.
- Source :
-
Fungal biology [Fungal Biol] 2011 Oct; Vol. 115 (10), pp. 959-70. Date of Electronic Publication: 2011 May 07. - Publication Year :
- 2011
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Abstract
- We report the first identification and characterisation of the glycerol-3-phosphate dehydrogenase (GPD) genes from extremely halophilic fungi. The black ascomycetous yeast Hortaea werneckii and the non-melanised basidiomycetous fungus Wallemia ichthyophaga inhabit similar hypersaline environments, yet they have two different strategies of haloadaptation through Gpd1-regulated glycerol synthesis. The extremely halotolerant H. werneckii codes for two salt-inducible GPD1 genes that show similar gene transcription regulation and have 98% amino-acid sequence identity between paralogues; however, they have distinct effects when expressed heterologously in Saccharomyces cerevisiae gpd mutants. Only the HwGpd1B isoform complements the function of Gpd in the gpd1 mutant, whereas none of the Gpd1 isoforms can rescue the salt sensitivity of the gpd1gpd2 double mutant. The obligate halophile W. ichthyophaga codes for only one GPD1 orthologue, the transcription of which is less affected by salt when compared to the H. werneckii homologues. Heterologous expression of WiGPD1 in S. cerevisiae recovers halotolerance of the gpd1 and gpd1gpd2 mutant strains, which is probably due to the overall high amino-acid similarity of the Gpd1 protein in W. ichthyophaga and S. cerevisiae. Phylogenetic analysis of amino-acid sequences reveals that the evolutionary origins of all of these three novel enzymes correspond to the phylogeny of the fungal species from which the genes were identified.<br /> (Copyright © 2011 British Mycological Society. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Ascomycota classification
Ascomycota genetics
Ascomycota isolation & purification
Basidiomycota classification
Basidiomycota genetics
Basidiomycota isolation & purification
Fungal Proteins chemistry
Fungal Proteins genetics
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Glycerolphosphate Dehydrogenase chemistry
Glycerolphosphate Dehydrogenase genetics
Molecular Sequence Data
Phylogeny
Salinity
Sequence Alignment
Ascomycota enzymology
Basidiomycota enzymology
Fungal Proteins metabolism
Glycerolphosphate Dehydrogenase metabolism
Seawater microbiology
Sodium Chloride metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-6146
- Volume :
- 115
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Fungal biology
- Publication Type :
- Academic Journal
- Accession number :
- 21944208
- Full Text :
- https://doi.org/10.1016/j.funbio.2011.04.001