Back to Search
Start Over
Differences between Trypanosoma brucei gambiense groups 1 and 2 in their resistance to killing by trypanolytic factor 1.
- Source :
-
PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2011 Sep; Vol. 5 (9), pp. e1287. Date of Electronic Publication: 2011 Sep 06. - Publication Year :
- 2011
-
Abstract
- Background: The three sub-species of Trypanosoma brucei are important pathogens of sub-Saharan Africa. T. b. brucei is unable to infect humans due to sensitivity to trypanosome lytic factors (TLF) 1 and 2 found in human serum. T. b. rhodesiense and T. b. gambiense are able to resist lysis by TLF. There are two distinct sub-groups of T. b. gambiense that differ genetically and by human serum resistance phenotypes. Group 1 T. b. gambiense have an invariant phenotype whereas group 2 show variable resistance. Previous data indicated that group 1 T. b. gambiense are resistant to TLF-1 due in-part to reduced uptake of TLF-1 mediated by reduced expression of the TLF-1 receptor (the haptoglobin-hemoglobin receptor (HpHbR)) gene. Here we investigate if this is also true in group 2 parasites.<br />Methodology: Isogenic resistant and sensitive group 2 T. b. gambiense were derived and compared to other T. brucei parasites. Both resistant and sensitive lines express the HpHbR gene at similar levels and internalized fluorescently labeled TLF-1 similar fashion to T. b. brucei. Both resistant and sensitive group 2, as well as group 1 T. b. gambiense, internalize recombinant APOL1, but only sensitive group 2 parasites are lysed.<br />Conclusions: Our data indicate that, despite group 1 T. b. gambiense avoiding TLF-1, it is resistant to the main lytic component, APOL1. Similarly group 2 T. b. gambiense is innately resistant to APOL1, which could be based on the same mechanism. However, group 2 T. b. gambiense variably displays this phenotype and expression does not appear to correlate with a change in expression site or expression of HpHbR. Thus there are differences in the mechanism of human serum resistance between T. b. gambiense groups 1 and 2.
- Subjects :
- Apolipoprotein L1
Apolipoproteins immunology
Cell Survival drug effects
Drug Resistance
Humans
Lipoproteins, HDL immunology
Parasitic Sensitivity Tests
Serum immunology
Serum parasitology
Trypanosoma brucei gambiense classification
Trypanosoma brucei gambiense immunology
Trypanosoma brucei gambiense physiology
Apolipoproteins pharmacology
Biological Products pharmacology
Lipoproteins, HDL pharmacology
Trypanosoma brucei gambiense drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1935-2735
- Volume :
- 5
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PLoS neglected tropical diseases
- Publication Type :
- Academic Journal
- Accession number :
- 21909441
- Full Text :
- https://doi.org/10.1371/journal.pntd.0001287