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Plasma appearance and disappearance of an oral dose of 25-hydroxyvitamin D2 in healthy adults.
- Source :
-
The British journal of nutrition [Br J Nutr] 2012 Apr; Vol. 107 (8), pp. 1128-37. Date of Electronic Publication: 2011 Sep 07. - Publication Year :
- 2012
-
Abstract
- 25-Hydroxyvitamin D (25(OH)D) half-life is a potential biomarker for investigating vitamin D metabolism and requirements. We performed a pilot study to assess the approach and practical feasibility of measuring 25(OH)D half-life after an oral dose. A total of twelve healthy Gambian men aged 18-23 years were divided into two groups to investigate the rate and timing of (1) absorption and (2) plasma disappearance after an 80 nmol oral dose of 25(OH)D2. Fasting blood samples were collected at baseline and, in the first group, every 2 h post-dose for 12 h, at 24 h, 48 h and on day 15. In the second group, fasting blood samples were collected on days 3, 4, 5, 6, 9, 12, 15, 18 and 21. Urine was collected for 2 h after the first morning void at baseline and on day 15. 25(OH)D2 plasma concentration was measured by ultra-performance liquid chromatography-tandem MS/MS and corrected for baseline. Biomarkers of vitamin D, Ca and P metabolism were measured at baseline and on day 15. The peak plasma concentration of 25(OH)D2 was 9·6 (sd 0·9) nmol/l at 4·4 (sd 1·8) h. The terminal slope of 25(OH)D2 disappearance was identified to commence from day 6. The terminal half-life of plasma 25(OH)D2 was 13·4 (sd 2·7) d. There were no significant differences in plasma 25(OH)D3, total 1,25(OH)2D, parathyroid hormone, P, Ca and ionised Ca and urinary Ca and P between baseline and day 15 and between the two groups. The present study provides data on the plasma response to oral 25(OH)D2 that will underpin and contribute to the further development of studies to investigate 25(OH)D half-life.
- Subjects :
- Administration, Oral
Adolescent
Biomarkers blood
Biomarkers urine
Calcium blood
Calcium urine
Creatinine urine
Half-Life
Humans
Intestinal Absorption
Male
Nutritional Requirements
Parathyroid Hormone blood
Phosphates blood
Phosphates urine
Tandem Mass Spectrometry
Young Adult
25-Hydroxyvitamin D 2 administration & dosage
25-Hydroxyvitamin D 2 blood
Subjects
Details
- Language :
- English
- ISSN :
- 1475-2662
- Volume :
- 107
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 21896243
- Full Text :
- https://doi.org/10.1017/S0007114511004132