A new model for the estimation of cell proliferation dynamics using CFSE data.

Authors :: Banks HT
Sutton KL
Thompson WC
Bocharov G
Doumic M
Schenkel T
Argilaguet J
Giest S
Peligero C
Meyerhans A
Source :: Journal of immunological methods [J Immunol Methods] 2011 Oct 28; Vol. 373 (1-2), pp. 143-60. Date of Electronic Publication: 2011 Aug 24.
Publication Year :: 2011
Abstract: CFSE analysis of a proliferating cell population is a popular tool for the study of cell division and divisionlinked changes in cell behavior. Recently Banks et al. (2011), Luzyanina et al. (2009), Luzyanina et al. (2007), a partial differential equation (PDE) model to describe lymphocyte dynamics in a CFSE proliferation assay was proposed. We present a significant revision of this model which improves the physiological understanding of several parameters. Namely, the parameter used previously as a heuristic explanation for the dilution of CFSE dye by cell division is replaced with a more physical component, cellular autofluorescence. The rate at which label decays is also quantified using a Gompertz decay process. We then demonstrate a revised method of fitting the model to the commonly used histogram representation of the data. It is shown that these improvements result in a model with a strong physiological basis which is fully capable of replicating the behavior observed in the data.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)