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Involvement of Arabidopsis NAC transcription factor in the regulation of 20S and 26S proteasomes.
- Source :
-
Plant science : an international journal of experimental plant biology [Plant Sci] 2011 Oct; Vol. 181 (4), pp. 421-7. Date of Electronic Publication: 2011 Jul 19. - Publication Year :
- 2011
-
Abstract
- We investigated the transcript levels of 13 proteasome subunit genes, the protein levels of proteasomes, and the activities of the 26S proteasome in ANAC078-overexpressing Arabidopsis plants (Ox-ANAC078) and knockout ANAC078 (KO-ANAC078) mutants. The transcript levels and the protein levels of proteasomes were increased in the Ox-ANAC078 plants compared with the wild-type plants and KO-ANAC078 mutants under normal conditions and high-light (HL) stress. Although the activities of the 26S proteasome were decreased in all the plants under HL stress, they were higher in the Ox-ANAC078 plants than wild-type plants and KO-ANAC078 mutants under normal conditions and HL stress. These findings suggest that ANAC078 regulates the levels of proteasomes. To explore the function of the increased levels of proteasomes to HL stress, we assessed the tolerance to HL stress of the Ox-ANAC078 plants and KO-ANAC078 mutants. The photosystem II activities of Ox-ANAC078 remained high compared with those of the wild-type plants and KO-ANAC078 mutants under HL stress, suggesting that ANAC078 may play an important role in the response and adaptation to HL stress.<br /> (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Arabidopsis drug effects
Arabidopsis genetics
Arabidopsis radiation effects
Arabidopsis Proteins genetics
Gene Expression Regulation, Plant drug effects
Gene Expression Regulation, Plant radiation effects
Gene Knockout Techniques
Genes, Plant genetics
Leupeptins pharmacology
Light
Mutation genetics
Photosystem II Protein Complex metabolism
Polyubiquitin metabolism
Proteasome Endopeptidase Complex genetics
Protein Subunits genetics
Protein Subunits metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Stress, Physiological drug effects
Stress, Physiological genetics
Stress, Physiological radiation effects
Transcription Factors genetics
Ubiquitination drug effects
Ubiquitination radiation effects
Arabidopsis enzymology
Arabidopsis Proteins metabolism
Proteasome Endopeptidase Complex metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2259
- Volume :
- 181
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant science : an international journal of experimental plant biology
- Publication Type :
- Academic Journal
- Accession number :
- 21889048
- Full Text :
- https://doi.org/10.1016/j.plantsci.2011.07.001