Back to Search
Start Over
Defining the anatomical localisation of subsets of the murine mononuclear phagocyte system using integrin alpha X (Itgax, CD11c) and colony stimulating factor 1 receptor (Csf1r, CD115) expression fails to discriminate dendritic cells from macrophages.
- Source :
-
Immunobiology [Immunobiology] 2011 Nov; Vol. 216 (11), pp. 1228-37. Date of Electronic Publication: 2011 Aug 17. - Publication Year :
- 2011
-
Abstract
- The murine mononuclear phagocyte (MNP) system comprises a diverse population of cells, including monocytes, dendritic cells (DC) and macrophages. Derived from the myeloid haematopoietic lineage, this group of cells express a variety of well characterized surface markers. Expression of the integrin alpha X (Itgax, CD11c) is commonly used to identify classical DC, and similarly expression of colony stimulating factor 1 receptor (Csf1r, CD115) to identify macrophages. We have characterized the expression of these markers using a variety of transgenic mouse models. We confirmed previous observations of Itgax expression in anatomically defined subsets of MNPs in secondary lymphoid organs, including all MNPs identified within the germinal centres. The majority of MNPs in the intestinal lamina propria and lung express Itgax. All mucosal Itgax expressing cells also express Csf1r suggesting Csf1-dependent haematopoietic derivation. This double-positive population included germinal centre MNPs. These data reveal that Itgax expression alone does not specifically define classical DC. These results suggest more cautious interpretation of Itgax-dependent experimentation and direct equation with uniquely DC-mediated activities, particularly in the functioning of non-lymphoid MNPs within the intestinal lamina propria.<br /> (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Biomarkers analysis
CD11c Antigen genetics
CD11c Antigen immunology
Cell Differentiation immunology
Cell Lineage immunology
Dendritic Cells cytology
Dendritic Cells immunology
Gene Expression
Immunohistochemistry
Immunophenotyping standards
Intestines cytology
Intestines immunology
Lung cytology
Lung immunology
Lymph Nodes cytology
Lymph Nodes immunology
Macrophage Colony-Stimulating Factor immunology
Macrophage Colony-Stimulating Factor metabolism
Macrophages cytology
Macrophages immunology
Mice
Mice, Transgenic
Monocytes cytology
Monocytes immunology
Mucous Membrane cytology
Mucous Membrane immunology
Receptor, Macrophage Colony-Stimulating Factor genetics
Receptor, Macrophage Colony-Stimulating Factor immunology
CD11c Antigen analysis
Dendritic Cells metabolism
Immunity, Innate
Immunophenotyping methods
Macrophages metabolism
Monocytes metabolism
Receptor, Macrophage Colony-Stimulating Factor analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3279
- Volume :
- 216
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 21885153
- Full Text :
- https://doi.org/10.1016/j.imbio.2011.08.006