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Phagocytosis of IgG-coated polystyrene beads by macrophages induces and requires high membrane order.
- Source :
-
Traffic (Copenhagen, Denmark) [Traffic] 2011 Dec; Vol. 12 (12), pp. 1730-43. Date of Electronic Publication: 2011 Sep 21. - Publication Year :
- 2011
-
Abstract
- The biochemical composition and biophysical properties of cell membranes are hypothesized to affect cellular processes such as phagocytosis. Here, we examined the plasma membranes of murine macrophage cell lines during the early stages of uptake of immunoglobulin G (IgG)-coated polystyrene particles. We found that the plasma membrane undergoes rapid actin-independent condensation to form highly ordered phagosomal membranes, the biophysical hallmark of lipid rafts. Surprisingly, these membranes are depleted of cholesterol and enriched in sphingomyelin and ceramide. Inhibition of sphingomyelinase activity impairs membrane condensation, F-actin accumulation at phagocytic cups and particle uptake. Switching phagosomal membranes to a cholesterol-rich environment had no effect on membrane condensation and the rate of phagocytosis. In contrast, preventing membrane condensation with the oxysterol 7-ketocholesterol, even in the presence of ceramide, blocked F-actin dissociation from nascent phagosomes and particle uptake. In conclusion, our results suggest that ordered membranes function to co-ordinate F-actin remodelling and that the biophysical properties of phagosomal membranes are essential for phagocytosis.<br /> (© 2011 John Wiley & Sons A/S.)
- Subjects :
- Actins metabolism
Animals
Cell Line
Cell Membrane immunology
Ceramides metabolism
Cholesterol metabolism
Coated Vesicles immunology
Coated Vesicles metabolism
Humans
Immunoglobulin G immunology
Macrophages immunology
Macrophages metabolism
Membrane Lipids metabolism
Mice
Monocytes immunology
Monocytes metabolism
Monocytes physiology
Phagocytosis immunology
Phagosomes immunology
Phagosomes metabolism
Phagosomes physiology
Sphingomyelin Phosphodiesterase metabolism
Sphingomyelins metabolism
Sterols metabolism
Cell Membrane metabolism
Cell Membrane physiology
Coated Vesicles physiology
Immunoglobulin G metabolism
Macrophages physiology
Phagocytosis physiology
Polystyrenes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0854
- Volume :
- 12
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Traffic (Copenhagen, Denmark)
- Publication Type :
- Academic Journal
- Accession number :
- 21883764
- Full Text :
- https://doi.org/10.1111/j.1600-0854.2011.01272.x