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Sphingomonas endophytica sp. nov., isolated from Artemisia annua L.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2012 Jul; Vol. 62 (Pt 7), pp. 1576-1580. Date of Electronic Publication: 2011 Aug 26. - Publication Year :
- 2012
-
Abstract
- A novel bacterium (strain YIM 65583(T)) belonging to the genus Sphingomonas was isolated from surface-sterilized tissue of Artemisia annua L., which was collected from Yunnan province, south-west China. Its morphology, physiology and biochemical features were consistent with those of members of the genus Sphingomonas. Analysis of the 16S rRNA gene sequence of strain YIM 65583(T) further confirmed that it should be classified as a member of the genus Sphingomonas and was most closely related to Sphingomonas phyllosphaerae FA2(T) (99.7%) and Sphingomonas yunnanensis YIM 003(T) (98.3%). The isolate was Gram-negative and formed yellow-pigmented colonies on ISP 2 medium. It grew optimally at pH 6.0-8.0, at 20-37 °C and in the presence of 0-1% (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-10; C(18:1)ω7c, anteiso-C(16:1), C(14:0)-2OH, C(17:1)ω6c, C(16:0) and C(15:0) were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The G+C content of the genomic DNA was 63.3 mol%. The DNA-DNA relatedness values of the isolate YIM 65583(T) with S. phyllosphaerae FA2(T) and S. yunnanensis YIM 003(T) were 43.1% and 37.9%, respectively. Based on these features, it is concluded that the strain represents a novel species of the genus Sphingomonas, for which the name Sphingomonas endophytica sp. nov. is proposed, with YIM 65583(T) (=CCTCC AA 209035(T)=JCM 17394(T)) as the type strain.
- Subjects :
- Bacterial Typing Techniques
Base Composition
China
Cluster Analysis
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fatty Acids analysis
Hydrogen-Ion Concentration
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids analysis
Phylogeny
Pigments, Biological metabolism
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Sodium Chloride metabolism
Sphingomonas genetics
Sphingomonas physiology
Temperature
Ubiquinone analysis
Artemisia annua microbiology
Sphingomonas classification
Sphingomonas isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 62
- Issue :
- Pt 7
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21873515
- Full Text :
- https://doi.org/10.1099/ijs.0.031484-0