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Relationship between oxidative stress and apoptotic markers in lymphocytes of diabetic patients with chronic non healing wound.
- Source :
-
Diabetes research and clinical practice [Diabetes Res Clin Pract] 2011 Dec; Vol. 94 (3), pp. 377-84. Date of Electronic Publication: 2011 Aug 26. - Publication Year :
- 2011
-
Abstract
- Aims: Hyperglycemia causes generation of free radicals which leads to oxidative stress and apoptosis in various cells. The present study was undertaken to investigate the correlation between oxidative stress and apoptotic markers in lymphocytes of diabetic patients with chronic non healing wounds.<br />Methods: Thirty healthy, thirty uncontrolled type 2 diabetes mellitus (T2DM) and thirty uncontrolled T2DM with chronic, non healing, neuropathic diabetic foot patients were included in this study. Indices of oxidative stress inside the lymphocyte lysate were estimated by measuring content of superoxide dismutase (SOD), Catalase, Glutathione and malonaldialdehyde (MDA). Protein expression studies of pro and anti apoptotic markers were carried out to elucidate their possible involvement in diabetic context.<br />Results: SOD and MDA activity was significantly higher in the lymphocytes of diabetic patients having chronic, non healing diabetic wound as compared with healthy (p<0.001); whereas catalase and GSH activity was significantly reduced (p<0.001) in the same group. Expressions of pro apoptotic markers (Caspase-3, Fas and Bax) were significantly higher whereas reduced expression of anti-apoptotic marker (Bcl-2) were obtained in lymphocytes of diabetic and non diabetic individuals.<br />Conclusions: Hyperglycemia confers pro apoptotic manifestations which are mostly through altered indices of oxidative stress within lymphocytic milieu.<br /> (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Blotting, Western
Case-Control Studies
Caspase 3 metabolism
Catalase metabolism
Chronic Disease
Diabetes Complications etiology
Diabetes Complications metabolism
Diabetes Complications pathology
Diabetic Foot etiology
Diabetic Foot metabolism
Diabetic Neuropathies etiology
Diabetic Neuropathies metabolism
Diabetic Neuropathies pathology
Glutathione metabolism
Glutathione Peroxidase metabolism
Humans
Hyperglycemia etiology
Hyperglycemia metabolism
Lymphocytes metabolism
Malondialdehyde metabolism
Middle Aged
Superoxide Dismutase metabolism
Thiobarbituric Acid Reactive Substances metabolism
Apoptosis
Biomarkers metabolism
Diabetes Mellitus, Type 2 physiopathology
Diabetic Foot pathology
Hyperglycemia pathology
Lymphocytes pathology
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8227
- Volume :
- 94
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Diabetes research and clinical practice
- Publication Type :
- Academic Journal
- Accession number :
- 21872354
- Full Text :
- https://doi.org/10.1016/j.diabres.2011.08.004