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Clinical efficacy of a specifically targeted antimicrobial peptide mouth rinse: targeted elimination of Streptococcus mutans and prevention of demineralization.
- Source :
-
Caries research [Caries Res] 2011; Vol. 45 (5), pp. 415-28. Date of Electronic Publication: 2011 Aug 19. - Publication Year :
- 2011
-
Abstract
- Background/aims: Streptococcus mutans, the major etiological agent of dental caries, has a measurable impact on domestic and global health care costs. Though persistent in the oral cavity despite conventional oral hygiene, S. mutans can be excluded from intact oral biofilms through competitive exclusion by other microorganisms. This suggests that therapies capable of selectively eliminating S. mutans while limiting the damage to the normal oral flora might be effective long-term interventions to fight cariogenesis. To meet this challenge, we designed C16G2, a novel synthetic specifically targeted antimicrobial peptide with specificity for S. mutans. C16G2 consists of a S. mutans-selective 'targeting region' comprised of a fragment from S. mutans competence stimulation peptide (CSP) conjoined to a 'killing region' consisting of a broad-spectrum antimicrobial peptide (G2). In vitro studies have indicated that C16G2 has robust efficacy and selectivity for S. mutans, and not other oral bacteria, and affects targeted bacteria within seconds of contact.<br />Methods: In the present study, we evaluated C16G2 for clinical utility in vitro, followed by a pilot efficacy study to examine the impact of a 0.04% (w/v) C16G2 rinse in an intra-oral remineralization/demineralization model.<br />Results and Conclusions: C16G2 rinse usage was associated with reductions in plaque and salivary S. mutans, lactic acid production, and enamel demineralization. The impact on total plaque bacteria was minimal. These results suggest that C16G2 is effective against S. mutans in vivo and should be evaluated further in the clinic.<br /> (Copyright © 2011 S. Karger AG, Basel.)
- Subjects :
- Adolescent
Adult
Aged
Animals
Anti-Infective Agents pharmacology
Antimicrobial Cationic Peptides chemical synthesis
Antimicrobial Cationic Peptides pharmacology
Bacterial Proteins chemical synthesis
Bacterial Proteins pharmacology
Biofilms drug effects
Cattle
Cell Culture Techniques
Cell Survival drug effects
Dental Plaque microbiology
Dental Plaque prevention & control
Gingiva cytology
Gingiva drug effects
Hemolysis drug effects
Humans
Hydrogen-Ion Concentration
Lactic Acid metabolism
Melitten pharmacology
Middle Aged
Mouth Mucosa cytology
Mouth Mucosa drug effects
Mouthwashes pharmacology
Pilot Projects
Saliva drug effects
Saliva microbiology
Species Specificity
Sucrose metabolism
Tooth Demineralization microbiology
Tooth Remineralization methods
Young Adult
Anti-Infective Agents therapeutic use
Antimicrobial Cationic Peptides therapeutic use
Bacterial Proteins therapeutic use
Mouthwashes therapeutic use
Streptococcus mutans drug effects
Tooth Demineralization prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1421-976X
- Volume :
- 45
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Caries research
- Publication Type :
- Academic Journal
- Accession number :
- 21860239
- Full Text :
- https://doi.org/10.1159/000330510