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Protein kinase C regulates ezrin-radixin-moesin phosphorylation in canine osteosarcoma cells.
- Source :
-
Veterinary and comparative oncology [Vet Comp Oncol] 2011 Sep; Vol. 9 (3), pp. 207-18. Date of Electronic Publication: 2010 Dec 14. - Publication Year :
- 2011
-
Abstract
- The development of metastasis is the most significant cause of death for both canine and human patients with osteosarcoma (OS). Ezrin has been associated with tumour progression and metastasis in human, canine and murine OS. Ezrin activation is dynamically regulated by protein kinase C (PKC) during metastatic progression in human and murine OS. To include the dog in the development of therapeutics that target ezrin biology, we characterized four new canine OS cell lines and confirmed the relationship between PKC and ezrin in these cells. Three of four cell lines formed tumours in mice that were histologically consistent with OS. All cell lines were markedly aneuploid and expressed ezrin and PKC. Finally, both ezrin phosphorylation and cell migration were inhibited using a PKC inhibitor. These data suggest that an association between PKC-mediated activation of ezrin and the metastatic phenotype in canine OS cells.<br /> (Published 2010. This article is a US Government work and is in the public domain in the USA.)
- Subjects :
- Animals
Bone Neoplasms metabolism
Cell Line, Tumor
Cell Movement
Dogs
Female
Membrane Proteins metabolism
Mice
Mice, SCID
Microfilament Proteins metabolism
Neoplasm Metastasis
Osteosarcoma metabolism
Phosphorylation
Signal Transduction
Wound Healing
Bone Neoplasms veterinary
Cytoskeletal Proteins metabolism
Dog Diseases metabolism
Osteosarcoma veterinary
Protein Kinase C metabolism
Rodent Diseases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5829
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Veterinary and comparative oncology
- Publication Type :
- Academic Journal
- Accession number :
- 21848623
- Full Text :
- https://doi.org/10.1111/j.1476-5829.2010.00249.x