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Characterization of a novel molluscan MyD88 family protein from manila clam, Ruditapes philippinarum.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2011 Dec; Vol. 31 (6), pp. 887-93. Date of Electronic Publication: 2011 Aug 10. - Publication Year :
- 2011
-
Abstract
- Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein which is required for signal transduction of TLR/IL-1R family. In this study, a novel molluscan MyD88 family member protein (named as RpMyD88) was identified from manila clam, Ruditapes philippinarum. It was identified using BLAST algorithm from GS-FLX™ sequencing data. The cDNA of RpMyD88 consists of 1416 bp open reading frame (ORF) encoding 471 amino acid residues. The RpMyD88 contains death domain and Toll/interleukin-1 receptor (TIR) domain which are typical features of MyD88 family proteins. The predicted amino acid sequence of RpMyD88 shares 27% identity with scallop MyD88. The expression level of RpMyD88 mRNA was investigated in healthy and challenged clams by quantitative real-time RT-PCR. The RpMyD88 gene expression is ubiquitous in all selected tissues. The RpMyD88 mRNA was strongly expressed in hemocyte, gill and mantle. In contrast, it was weakly expressed in siphon, foot and adductor muscle. RpMyD88 was up-regulated in gill and hemocyte after immune challenge with both Vibrio tapetis and LPS challenge. All results considered, sequence characterization, comparison and gene expression data suggesting that MyD88-dependent signaling pathway is presence in manila clam and RpMyD88 plays an important role in innate immune response against bacteria.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Computational Biology
DNA Primers genetics
DNA, Complementary genetics
Gene Expression Profiling
Gene Expression Regulation drug effects
Lipopolysaccharides pharmacology
Molecular Sequence Data
Open Reading Frames genetics
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology
Bivalvia genetics
Gene Expression Regulation immunology
Myeloid Differentiation Factor 88 genetics
Vibrio immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 31
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 21846503
- Full Text :
- https://doi.org/10.1016/j.fsi.2011.08.003