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Gene expression profiles of immune mediators and histopathological findings in animal models of leptospirosis: comparison between susceptible hamsters and resistant mice.
- Source :
-
Infection and immunity [Infect Immun] 2011 Nov; Vol. 79 (11), pp. 4480-92. Date of Electronic Publication: 2011 Aug 15. - Publication Year :
- 2011
-
Abstract
- Leptospirosis is a widespread zoonosis characterized by multiple organ failure and variable host susceptibility toward pathogenic Leptospira strains. In this study, we put the role of inflammatory mediators in parallel with bacterial burdens and organ lesions by comparing a susceptible animal model, the hamster, and a resistant one, the Oncins France 1 (OF1) mouse, both infected with virulent Leptospira interrogans serovar Icterohaemorrhagiae strain Verdun. Histological observations evidenced edema, congestion, hemorrhage, and inflammatory infiltration in the organs of hamsters, in contrast to limited changes in mice. Using reverse transcription-quantitative PCR techniques, we showed that the relative Leptospira burden progressively increased in hamster tissues, while a rapid clearance was observed in mouse tissues. The early regulation of the proinflammatory mediators interleukin-1β (IL-1β), IL-6, tumor necrosis factor alpha, and cyclo-oxygenase-2 and the chemokines gamma interferon-inducible protein 10 kDa/CXCL10 and macrophage inflammatory protein-1α/CCL3 in mouse tissues contrasted with their delayed and massive overexpression in hamster tissues. Conversely, the induction of the anti-inflammatory cytokine IL-10 was faster in the resistant than in the susceptible animal model. The role of these cytokines in the pathophysiology of leptospirosis and the implications of their differential regulation in the development of this disease are discussed.
- Subjects :
- Animals
Cricetinae
Cytokines genetics
Cytokines metabolism
Disease Models, Animal
Disease Susceptibility
Gene Expression Regulation immunology
Kidney pathology
Leptospirosis mortality
Liver pathology
Lung pathology
Mesocricetus
Mice
RNA, Messenger genetics
RNA, Messenger metabolism
Gene Expression Profiling
Leptospira interrogans serovar icterohaemorrhagiae immunology
Leptospirosis immunology
Leptospirosis pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 79
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 21844232
- Full Text :
- https://doi.org/10.1128/IAI.05727-11