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An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells.
- Source :
-
Nature biotechnology [Nat Biotechnol] 2011 Aug 14; Vol. 29 (9), pp. 829-34. Date of Electronic Publication: 2011 Aug 14. - Publication Year :
- 2011
-
Abstract
- An important risk in the clinical application of human pluripotent stem cells (hPSCs), including human embryonic and induced pluripotent stem cells (hESCs and hiPSCs), is teratoma formation by residual undifferentiated cells. We raised a monoclonal antibody against hESCs, designated anti-stage-specific embryonic antigen (SSEA)-5, which binds a previously unidentified antigen highly and specifically expressed on hPSCs--the H type-1 glycan. Separation based on SSEA-5 expression through fluorescence-activated cell sorting (FACS) greatly reduced teratoma-formation potential of heterogeneously differentiated cultures. To ensure complete removal of teratoma-forming cells, we identified additional pluripotency surface markers (PSMs) exhibiting a large dynamic expression range during differentiation: CD9, CD30, CD50, CD90 and CD200. Immunohistochemistry studies of human fetal tissues and bioinformatics analysis of a microarray database revealed that concurrent expression of these markers is both common and specific to hPSCs. Immunodepletion with antibodies against SSEA-5 and two additional PSMs completely removed teratoma-formation potential from incompletely differentiated hESC cultures.
- Subjects :
- Animals
Biomarkers
Cell Differentiation
Cells, Cultured
Computational Biology
Flow Cytometry
Gene Expression Regulation
Humans
Immunohistochemistry
Mice
Mice, Inbred BALB C
Microarray Analysis
Pluripotent Stem Cells chemistry
Real-Time Polymerase Chain Reaction
Teratoma metabolism
Antibodies, Monoclonal metabolism
Pluripotent Stem Cells cytology
Polysaccharides metabolism
Stage-Specific Embryonic Antigens metabolism
Teratoma pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1546-1696
- Volume :
- 29
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Nature biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 21841799
- Full Text :
- https://doi.org/10.1038/nbt.1947