Back to Search
Start Over
Actin dynamics counteract membrane tension during clathrin-mediated endocytosis.
- Source :
-
Nature cell biology [Nat Cell Biol] 2011 Aug 14; Vol. 13 (9), pp. 1124-31. Date of Electronic Publication: 2011 Aug 14. - Publication Year :
- 2011
-
Abstract
- Clathrin-mediated endocytosis is independent of actin dynamics in many circumstances but requires actin polymerization in others. We show that membrane tension determines the actin dependence of clathrin-coat assembly. As found previously, clathrin assembly supports formation of mature coated pits in the absence of actin polymerization on both dorsal and ventral surfaces of non-polarized mammalian cells, and also on basolateral surfaces of polarized cells. Actin engagement is necessary, however, to complete membrane deformation into a coated pit on apical surfaces of polarized cells and, more generally, on the surface of any cell in which the plasma membrane is under tension from osmotic swelling or mechanical stretching. We use these observations to alter actin dependence experimentally and show that resistance of the membrane to propagation of the clathrin lattice determines the distinction between 'actin dependent and 'actin independent'. We also find that light-chain-bound Hip1R mediates actin engagement. These data thus provide a unifying explanation for the role of actin dynamics in coated-pit budding.
- Subjects :
- Adaptor Protein Complex 2 genetics
Adaptor Protein Complex 2 metabolism
Aminoquinolines pharmacology
Animals
Benzazepines pharmacology
Bridged Bicyclo Compounds, Heterocyclic pharmacology
Cell Line
Cell Membrane drug effects
Cell Membrane ultrastructure
Cell Polarity
Clathrin-Coated Vesicles drug effects
Clathrin-Coated Vesicles ultrastructure
Coated Pits, Cell-Membrane drug effects
Coated Pits, Cell-Membrane metabolism
Coated Pits, Cell-Membrane ultrastructure
Cytoskeletal Proteins genetics
Cytoskeletal Proteins metabolism
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Microscopy, Electron
Microscopy, Fluorescence
Osmotic Pressure
Oximes pharmacology
Pyrimidines pharmacology
RNA Interference
Stress, Mechanical
Thiazolidines pharmacology
Actins metabolism
Cell Membrane metabolism
Clathrin metabolism
Clathrin-Coated Vesicles metabolism
Endocytosis
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4679
- Volume :
- 13
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Nature cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 21841790
- Full Text :
- https://doi.org/10.1038/ncb2307