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Ptf1a/Rbpj complex inhibits ganglion cell fate and drives the specification of all horizontal cell subtypes in the chick retina.
- Source :
-
Developmental biology [Dev Biol] 2011 Oct 15; Vol. 358 (2), pp. 296-308. Date of Electronic Publication: 2011 Jul 31. - Publication Year :
- 2011
-
Abstract
- During development, progenitor cells of the retina give rise to six principal classes of neurons and the Müller glial cells found within the adult retina. The pancreas transcription factor 1 subunit a (Ptf1a) encodes a basic-helix-loop-helix transcription factor necessary for the specification of horizontal cells and the majority of amacrine cell subtypes in the mouse retina. The Ptf1a-regulated genes and the regulation of Ptf1a activity by transcription cofactors during retinogenesis have been poorly investigated. Using a retrovirus-mediated gene transfer approach, we reported that Ptf1a was sufficient to promote the fates of amacrine and horizontal cells from retinal progenitors and inhibit retinal ganglion cell and photoreceptor differentiation in the chick retina. Both GABAergic H1 and non-GABAergic H3 horizontal cells were induced following the forced expression of Ptf1a. We describe Ptf1a as a strong, negative regulator of Atoh7 expression. Furthermore, the Rbpj-interacting domains of Ptf1a protein were required for its effects on cell fate specification. Together, these data provide a novel insight into the molecular basis of Ptf1a activity on early cell specification in the chick retina.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Avian Proteins chemistry
Avian Proteins genetics
Basic Helix-Loop-Helix Transcription Factors chemistry
Basic Helix-Loop-Helix Transcription Factors genetics
Cell Differentiation
Chick Embryo
Gene Expression Regulation, Developmental
Immunoglobulin J Recombination Signal Sequence-Binding Protein chemistry
Immunoglobulin J Recombination Signal Sequence-Binding Protein genetics
Mice
Multiprotein Complexes
Protein Interaction Domains and Motifs
Retina cytology
Retinal Ganglion Cells classification
Transcription Factors chemistry
Transcription Factors genetics
Avian Proteins metabolism
Basic Helix-Loop-Helix Transcription Factors metabolism
Immunoglobulin J Recombination Signal Sequence-Binding Protein metabolism
Retina embryology
Retina metabolism
Retinal Ganglion Cells cytology
Retinal Ganglion Cells metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-564X
- Volume :
- 358
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 21839069
- Full Text :
- https://doi.org/10.1016/j.ydbio.2011.07.033