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Protease activation during HIV infection in a CD4-positive cell line.
- Source :
-
AIDS research and human retroviruses [AIDS Res Hum Retroviruses] 1990 Feb; Vol. 6 (2), pp. 251-60. - Publication Year :
- 1990
-
Abstract
- The mechanism of cytopathic effects associated with HIV infection in a continuous line of CD4-positive lymphocytes (CEM cells, clone 13) has been studied. Here we report the following observations: (1) HIV infection killed a variable but always significant number of cells without a strict relationship with the syncytia formation; (2) an important decrease in the proliferation rate occurred soon after infection; (3) a marked inhibition of protein synthesis took place within the first few hours of infection and clearly before the beginning of viral protein expression. In addition, when three-day-old cultures were incubated in serum-free medium, a larger degradation of proteins was observed in infected cells in comparison to controls. An increase in protein degradation activity was observed also in vitro with extracts obtained from HIV-infected cells and incubated in the presence of endogenous- or exogenous-labeled substrates. Extracts from cells infected with heat-inactivated HIV did not show a similar degradative activity. The possible induction or activation of latent proteases during the development of the HIV infection is discussed.
- Subjects :
- CD4 Antigens immunology
Cell Survival
Child, Preschool
Cytopathogenic Effect, Viral
Enzyme Activation drug effects
HIV Protease
HIV-1 immunology
HIV-1 physiology
Humans
Kinetics
Leukocyte Count
Protease Inhibitors pharmacology
Protein Denaturation
Tumor Cells, Cultured
Endopeptidases biosynthesis
Gene Products, pol biosynthesis
HIV Infections enzymology
HIV-1 enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0889-2229
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- AIDS research and human retroviruses
- Publication Type :
- Academic Journal
- Accession number :
- 2183854
- Full Text :
- https://doi.org/10.1089/aid.1990.6.251