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Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1990 Jan 25; Vol. 18 (2), pp. 355-9. - Publication Year :
- 1990
-
Abstract
- The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed.
- Subjects :
- Amino Acid Sequence
Base Sequence
Cloning, Molecular methods
Electrophoresis, Polyacrylamide Gel
Enterobacter enzymology
Escherichia coli enzymology
Escherichia coli genetics
Molecular Sequence Data
Molecular Weight
Plasmids
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Restriction Mapping
Site-Specific DNA-Methyltransferase (Adenine-Specific) isolation & purification
Site-Specific DNA-Methyltransferase (Adenine-Specific) metabolism
Substrate Specificity
Enterobacter genetics
Enterobacteriaceae genetics
Genes, Bacterial
Site-Specific DNA-Methyltransferase (Adenine-Specific) genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 2183182
- Full Text :
- https://doi.org/10.1093/nar/18.2.355