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Conantokin-T. A gamma-carboxyglutamate containing peptide with N-methyl-d-aspartate antagonist activity.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1990 Apr 15; Vol. 265 (11), pp. 6025-9. - Publication Year :
- 1990
-
Abstract
- Conantokin-T, a 21-amino acid peptide which induces sleep-like symptoms in young mice was purified from the venom of the fish-hunting cone snail, Conus tulipa. The amino acid sequence of the peptide was determined and verified by chemical synthesis. The peptide has 4 residues of the modified amino acid, gamma-carboxyglutamate (Gla). The sequence of the peptide is: Gly-Glu-Gla-Gla-Tyr-Gln-Lys-Met-Leu-Gla-Asn-Leu-Arg-Gla-Ala-Glu-Val-Lys- Lys-Asn-Ala-NH2. Conantokin-T inhibits N-methyl-D-aspartate (NMDA) receptor-mediated calcium influx in central nervous system neurons. This observation suggests that like conantokin-G (a homologous Conus peptide with recently identified NMDA antagonist activity) conantokin-T has NMDA antagonist activity. A sequence comparison of conantokins-T and -G identifies the 4 Gla residues and the N-terminal dipeptide sequence as potential key elements for the biological activity of this peptide.
- Subjects :
- Amino Acid Sequence
Animals
Aspartic Acid antagonists & inhibitors
Aspartic Acid pharmacology
Benzofurans
Calcium metabolism
Cerebellum drug effects
Cerebellum metabolism
Chromatography, Gel
Conotoxins
Fluorescent Dyes
Fura-2
Intercellular Signaling Peptides and Proteins
Mass Spectrometry
Mice
Molecular Sequence Data
Mollusk Venoms chemical synthesis
Mollusk Venoms pharmacology
N-Methylaspartate
Neurons drug effects
Neurons metabolism
Peptides chemical synthesis
Peptides pharmacology
Rats
Seizures chemically induced
Sequence Homology, Nucleic Acid
Snails
1-Carboxyglutamic Acid analysis
Aspartic Acid analogs & derivatives
Mollusk Venoms isolation & purification
Peptides isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 265
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2180939