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Elsholtzia ciliata inhibits mast cell-mediated allergic inflammation: role of calcium, p38 mitogen-activated protein kinase and nuclear factor-{kappa}B.
- Source :
-
Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2011 Sep; Vol. 236 (9), pp. 1070-7. Date of Electronic Publication: 2011 Aug 01. - Publication Year :
- 2011
-
Abstract
- Mast cell-mediated allergic reaction is involved in many diseases such as asthma and allergic rhinitis. Therefore, discovery of drugs for the prevention or treatment of allergic disease is an important topic in human health. In this study, we evaluated the effects of water extract of Elsholtzia ciliata (Thunb.) Hyland (Labiatae) (WEEC) on mast cell-mediated allergic inflammation and studied the possible mechanisms of action. WEEC inhibited compound 48/80-induced systemic and immunoglobulin E-mediated local anaphylaxis, and serum histamine release in mice. WEEC reduced intracellular calcium levels and downstream histamine release from human mast cells (HMC-1) activated with phorbol 12-myristate 13-acetate and calcium ionophore A23187. In addition, WEEC decreased gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in HMC-1. The inhibitory effect of WEEC on cytokine expression was nuclear factor (NF)-κB and p38 mitogen-activated protein kinase (MAPK) dependent. Our results indicate that WEEC inhibits mast cell-mediated allergic inflammatory reactions by suppressing histamine release and proinflammatory cytokine expression, and involvement of calcium, NF-κB and p38 MAPK in these effects.
- Subjects :
- Animals
Calcium blood
Histamine Release drug effects
Hypersensitivity physiopathology
Inflammation drug therapy
Inflammation physiopathology
Male
Mast Cells physiology
Mice
Mice, Inbred ICR
NF-kappa B drug effects
Passive Cutaneous Anaphylaxis drug effects
Rats
Rats, Sprague-Dawley
p-Methoxy-N-methylphenethylamine pharmacology
p38 Mitogen-Activated Protein Kinases drug effects
Calcium physiology
Hypersensitivity drug therapy
Lamiaceae
Mast Cells drug effects
NF-kappa B physiology
Plant Extracts pharmacology
p38 Mitogen-Activated Protein Kinases physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3699
- Volume :
- 236
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Experimental biology and medicine (Maywood, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 21807818
- Full Text :
- https://doi.org/10.1258/ebm.2011.011017