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Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression.
- Source :
-
Nature biotechnology [Nat Biotechnol] 2011 Jul 31; Vol. 29 (8), pp. 742-9. Date of Electronic Publication: 2011 Jul 31. - Publication Year :
- 2011
-
Abstract
- Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer-associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencing of polyA(+) RNA (RNA-Seq) from a cohort of 102 prostate tissues and cells lines. We characterized one ncRNA, PCAT-1, as a prostate-specific regulator of cell proliferation and show that it is a target of the Polycomb Repressive Complex 2 (PRC2). We further found that patterns of PCAT-1 and PRC2 expression stratified patient tissues into molecular subtypes distinguished by expression signatures of PCAT-1-repressed target genes. Taken together, our findings suggest that PCAT-1 is a transcriptional repressor implicated in a subset of prostate cancer patients. These findings establish the utility of RNA-Seq to identify disease-associated ncRNAs that may improve the stratification of cancer subtypes.
- Subjects :
- Base Sequence
Biomarkers, Tumor metabolism
Cell Growth Processes physiology
Cluster Analysis
Cohort Studies
Computational Biology
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Disease Progression
Enhancer of Zeste Homolog 2 Protein
Humans
Male
Molecular Sequence Data
Polycomb Repressive Complex 2
Polycomb-Group Proteins
Prostatic Neoplasms metabolism
Prostatic Neoplasms pathology
RNA, Untranslated metabolism
Repressor Proteins genetics
Repressor Proteins metabolism
Reproducibility of Results
Signal Transduction
Transcription Factors genetics
Transcription Factors metabolism
Transcriptome
Biomarkers, Tumor genetics
Prostatic Neoplasms genetics
RNA, Untranslated genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1546-1696
- Volume :
- 29
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Nature biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 21804560
- Full Text :
- https://doi.org/10.1038/nbt.1914