Back to Search
Start Over
Evaluation of protein expression in bovine bronchoalveolar fluid following challenge with Mannheimia haemolytica.
- Source :
-
Proteomics [Proteomics] 2011 Sep; Vol. 11 (18), pp. 3685-97. Date of Electronic Publication: 2011 Aug 23. - Publication Year :
- 2011
-
Abstract
- Proteomics analysis of bovine bronchoalveolar fluid (BAF) following induction of pneumonia with Mannheimia haemolytica using nanoflow liquid chromatography coupled with tandem mass spectrometry (nanoLC-MS/MS) resulted in the identification of 88 unique proteins. Proteins detected in BAF included antimicrobial peptides (AMPs), complement factors, acute-phase proteins, protease inhibitors, and proteins involved in oxidation-reduction. Notwithstanding biological variation, differences in relative protein abundance, determined using normalized peptide counts, were detected for select proteins in BAF from genuinely infected versus sham-infected animals. To demonstrate the applicability of using normalized peptide counts to assess protein expression trends, LC-MS/MS data for the acute-phase protein haptoglobin (HPT) were compared with ELISA data, and statistical evaluation of the relationship between the data revealed a strong measure of association. Differences were detected between sham- and genuinely infected animals for haptoglobin, as well as the AMPs cathelicidin-1 and cathelicidin-4, and inter-α-trypsin inhibitor heavy chain-4, a fairly novel protein involved in the acute phase response. Though the small sample size limited the scope of the inferences, the results indicate the likely importance of AMPs and acute-phase proteins during respiratory infection, and provide additional information regarding potential mechanisms involved in the bovine mucosal barrier defense.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Animals
Bronchoalveolar Lavage Fluid immunology
Bronchoalveolar Lavage Fluid microbiology
Cathelicidins analysis
Cattle microbiology
Cattle Diseases immunology
Cattle Diseases microbiology
Chromatography, Liquid
Enzyme-Linked Immunosorbent Assay
Haptoglobins analysis
Male
Mannheimia haemolytica immunology
Peptide Fragments analysis
Pneumonia of Calves, Enzootic immunology
Pneumonia of Calves, Enzootic microbiology
Proteomics
Tandem Mass Spectrometry
Bronchoalveolar Lavage Fluid chemistry
Cattle metabolism
Mannheimia haemolytica pathogenicity
Proteome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9861
- Volume :
- 11
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 21800424
- Full Text :
- https://doi.org/10.1002/pmic.201000710