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Chemiluminescence enzyme immunoassay for the determination of sulfamethoxydiazine.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2011 Oct 15; Vol. 81 (1), pp. 544-7. Date of Electronic Publication: 2011 Jul 06. - Publication Year :
- 2011
-
Abstract
- Sulfamethoxydiazine (SMD), which is often used for animal disease treatment, is harmful to human health. No SMD residue should be detected in food in some countries, such as USA and Japan. Therefore, it is significant to develop a high-throughput, high-sensitivity and accurate method for the determination of the content of SMD in food. In this paper, chemiluminescence enzyme immunoassay (CLEIA) was developed for quantification of SMD. For this method, the limit of detection was 3.2 pg/ml, the linear range was from 10 to 2000 pg/ml, the within-day and inter-day precision were below 13% and below 18%, respectively, and the recovery was from 85% to 105%. Milk and egg were selected as samples to be examined with this method, and the result indicated that this CLEIA method was suitable for screening and quality control of food.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Anti-Infective Agents analysis
Calibration
Eggs analysis
Food Analysis methods
Food Contamination analysis
High-Throughput Screening Assays methods
High-Throughput Screening Assays standards
Humans
Immunoenzyme Techniques methods
Immunoenzyme Techniques standards
Limit of Detection
Luminescent Measurements standards
Milk chemistry
Sensitivity and Specificity
Luminescent Measurements methods
Sulfameter analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 81
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 21795101
- Full Text :
- https://doi.org/10.1016/j.saa.2011.06.047