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Transduced Tat-Annexin protein suppresses inflammation-associated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells.
- Source :
-
BMB reports [BMB Rep] 2011 Jul; Vol. 44 (7), pp. 484-9. - Publication Year :
- 2011
-
Abstract
- Annexin-1 (ANX1) is an anti-inflammatory protein as well as an important modulator in inflammation. However, the precise action of ANX1 remains unclear. To elucidate the protective effects of ANX1 on lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells, we constructed a cell-permeable Tat-ANX1 protein. The transduced Tat-ANX1 protein markedly inhibited the expression of cyclooxygenase-2, production of prostaglandin E(2), and generation of pro-inflammatory cytokines in the cells. Furthermore, transduced Tat-ANX1 protein caused a significant reduction in the activation of nuclear factor- kappa B (NF-kB) and mitogen-activated protein kinase (MAPK). The results indicate that Tat-ANX1 inhibits the production of inflammatory response cytokines and enzymes by blocking NF-kB and MAPK. Therefore, Tat-ANX1 protein may be useful as a therapeutic agent against various inflammatory diseases.
- Subjects :
- Animals
Cell Line
Cyclooxygenase 2 metabolism
Cytokines metabolism
Dinoprostone biosynthesis
Enzyme Activation drug effects
Humans
Mice
Mitogen-Activated Protein Kinases metabolism
NF-kappa B metabolism
Recombinant Fusion Proteins pharmacology
Annexins metabolism
Gene Expression Regulation drug effects
Inflammation genetics
Lipopolysaccharides pharmacology
Recombinant Fusion Proteins metabolism
Transduction, Genetic
tat Gene Products, Human Immunodeficiency Virus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 44
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Academic Journal
- Accession number :
- 21777521
- Full Text :
- https://doi.org/10.5483/BMBRep.2011.44.7.484